首次在伊朗从土壤中分离出……（原文“from soil in Iran”前缺关键信息，此译文为根据现有内容的合理补充翻译）

The first isolation of from soil in Iran.

作者信息

Jamali S, Gharaei M

机构信息

Assistant Professor of Mycology, Department of Plant Protection, School of Agriculture, Razi University, Kermanshah, Iran.

MS student, Department of Plant Protection, School of Agriculture, Razi University, Kermanshah, Iran.

出版信息

Curr Med Mycol. 2015 Sep;1(3):3-10. doi: 10.18869/acadpub.cmm.1.3.3.

DOI:10.18869/acadpub.cmm.1.3.3

PMID:28680990

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5490323/

Abstract

BACKGROUND AND PURPOSE

is a genus of anamorphic basidiomycetous yeast which is widely distributed in nature and is found in tropical and temperate areas. The aim of this work was to study the isolation, identification and molecular analysis of species in soil.

MATERIALS AND METHODS

In order to isolate and identify species in soil, 30 samples were collected from 30 different locations across Iran. The isolates were identified by means of the standard methods of yeast identification. To confirm morphological identification, genomic DNA was extracted and the hypervariable D1/D2 domain of the large-subunit (LSU) ribosomal DNA (rDNA) gene was amplified by polymerase chain reaction (PCR), using primer pair NL-1/NL-4, and then the sequences were analyzed.

RESULTS

According to the morphological and physiological assessments, isolates were identified as . The isolates formed chlamydospore after one week on yeast-malt (YM) agar medium. Using Blast program, we found that the D1/D2 sequences of the isolates from Iran (accession no: KP055040 and KP055041) showed 99% homology with the deposited in GenBank. All the isolates placed in the Ovoides cluster were well-supported by bootstrap values.

CONCLUSION

The present study is the first attempt to survey in soil of Iran. To the best of our knowledge, this is the first investigation of in Iran.

摘要

背景与目的

[具体菌名]是一种无性型担子菌酵母属，广泛分布于自然界，在热带和温带地区均有发现。本研究的目的是对土壤中的[具体菌名]进行分离、鉴定及分子分析。

材料与方法

为了分离和鉴定土壤中的[具体菌名]，从伊朗各地30个不同地点采集了30份样本。采用酵母鉴定的标准方法对分离株进行鉴定。为了确认形态学鉴定结果，提取了基因组DNA，并使用引物对NL-1/NL-4通过聚合酶链反应（PCR）扩增大亚基（LSU）核糖体DNA（rDNA）基因的高变D1/D2结构域，然后对序列进行分析。

结果

根据形态学和生理学评估，分离株被鉴定为[具体菌名]。分离株在酵母麦芽（YM）琼脂培养基上培养一周后形成厚垣孢子。使用Blast程序，我们发现来自伊朗的[具体菌名]分离株（登录号：KP055040和KP055041）的D1/D2序列与GenBank中登记的[具体菌名]序列显示出99%的同源性。所有归入卵形簇的[具体菌名]分离株都得到了自展值的有力支持。