Ghamiloui M M, Valadkhani Z, Rahimi F
Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran.
Cornea Clinic, Farabi Research Eye Hospital, Professor of ophthalmology, Tehran University of Medical Science, Tehran, Iran.
Curr Med Mycol. 2015 Sep;1(3):39-44. doi: 10.18869/acadpub.cmm.1.3.39.
Microsporidiosis is one of the emerging and opportunistic infections, which causing various clinical symptoms in humans. The prevalence of this infection varies, depending on the infected organ, diagnostic methods, and geographical conditions. In the present study, we aimed to investigate microsporidial keratitis in patients referring to Farabi Eye Hospital Tehran, Iran in 2013-14.
Two scraping samples were collected from 91 keratitis patients, five cases had prior history of receiving immune suppressive drugs. One of the two collected samples from each participant was used for Vero cell culture and the other was used for the preparation of Giemsa and Gram staining slides. After 30 days, the cells were scrapped and used for DNA extraction; afterwards, nested polymerase chain reaction (PCR) detection method was applied. Primer pairs of small-subunit ribosomal RNA gene were designed by CLC Genomics workbench software to amplify all major microsporidian pathogens, as well as , which was used as the positive control in this study.
The nested PCR showed negative results regarding the presence of microsporidia in the samples. Similarly, Giemsa and Gram staining slides did not detect any spores.
The prevalence of human microsporidiosis ranges between 0% and 50%, worldwide. Based on all the negative samples in the present study, we can conclude that the prevalence of this infection among Iranian patients falls in the lower quartile. By gathering further evidence, researchers can take a step forward in this area and open new doors for the assessment of AIDS patients and users of immunosuppressive drugs.
微孢子虫病是一种新出现的机会性感染疾病，可在人类身上引发各种临床症状。这种感染的患病率因感染器官、诊断方法和地理条件而异。在本研究中，我们旨在调查2013 - 2014年转诊至伊朗德黑兰法拉比眼科医院的患者中的微孢子虫性角膜炎情况。
从91例角膜炎患者中采集两份刮片样本，其中5例有接受免疫抑制药物的既往史。从每位参与者采集的两份样本中，一份用于Vero细胞培养，另一份用于制备吉姆萨染色和革兰氏染色玻片。30天后，刮取细胞用于DNA提取；随后，应用巢式聚合酶链反应（PCR）检测方法。通过CLC基因组学工作台软件设计小亚基核糖体RNA基因的引物对，以扩增所有主要的微孢子虫病原体，以及 ，其在本研究中用作阳性对照。
巢式PCR显示样本中不存在微孢子虫，结果为阴性。同样，吉姆萨染色和革兰氏染色玻片未检测到任何孢子。
在全球范围内，人类微孢子虫病的患病率在0%至50%之间。基于本研究中所有样本均为阴性的结果，我们可以得出结论，这种感染在伊朗患者中的患病率处于较低四分位数。通过收集更多证据，研究人员可以在该领域向前迈进一步，为评估艾滋病患者和免疫抑制药物使用者打开新的大门。
