Charité Center for Internal Medicine and Dermatology, Department for Psychosomatic Medicine, Charite - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, Berlin, Germany.
Charité Center for Internal Medicine and Dermatology, Department for Psychosomatic Medicine, Charite - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, Berlin, Germany; Department of Internal Medicine, HELIOS Kliniken GmbH, Rottweil, Germany; Department of Psychosomatic Medicine and Psychotherapy, University Hospital Tübingen, Tübingen, Germany.
Brain Res. 2019 Jul 15;1715:188-195. doi: 10.1016/j.brainres.2019.03.034. Epub 2019 Mar 28.
Phoenixin is a novel neuropeptide initially associated with reproductive functions, but subsequently also with feeding behavior. Nesfatin-1 is also involved in the regulation of food intake and has been shown to largely colocalize with phoenixin in the rat brain; however, a functional link is missing so far. The current study investigated whether phoenixin activates nesfatin-1 immunoreactive nuclei in the rat brain. Male Sprague Dawley rats chronically equipped with an intracerebroventricular cannula were injected with vehicle (5 µl ddHO) or phoenixin (1.7 nmol in 5 µl ddHO, n = 5-6 group). Behavior was assessed manually and c-Fos as well as nesfatin-1 immunoreactivity using immunohistochemistry. Phoenixin significantly increased feeding and drinking behavior as well as locomotor activity compared to vehicle (p < 0.01). Moreover, phoenixin injected intracerebroventricularly (icv) activated several nuclei throughout the rat brain as assessed using c-Fos; the number of c-Fos/nesfatin-1 immunoreactive neurons was increased in the lateral septal nucleus (4-fold), supraoptic nucleus (107-fold), paraventricular nucleus (6-fold) and the nucleus of the solitary tract (18-fold) compared to vehicle (p < 0.05). In summary, phoenixin activates several nesfatin-1 immunoreactive nuclei in the rat brain. This activation may play a role in the modulation of food intake.
凤凰素是一种新型神经肽，最初与生殖功能有关，但随后也与摄食行为有关。 nesfatin-1 也参与了摄食行为的调节，并且已经表明它在大鼠脑中与凤凰素大量共定位；然而，目前还缺乏功能联系。本研究探讨了凤凰素是否能激活大鼠脑中的 nesfatin-1 免疫反应性核。雄性 Sprague Dawley 大鼠长期配备有侧脑室套管，用载体（5μl ddHO）或凤凰素（1.7 nmol 在 5μl ddHO 中，n=5-6 组）注射。通过手动评估行为以及通过免疫组织化学评估 c-Fos 和 nesfatin-1 免疫反应性。与载体相比，凤凰素显著增加了摄食和饮水行为以及运动活性（p<0.01）。此外，用 c-Fos 评估发现，凤凰素经脑室内（icv）注射可激活大鼠大脑中的多个核，与载体相比，外侧隔核（4 倍）、视上核（107 倍）、室旁核（6 倍）和孤束核（18 倍）的 c-Fos/nesfatin-1 免疫反应性神经元数量增加（p<0.05）。总之，凤凰素激活了大鼠脑中的几个 nesfatin-1 免疫反应性核。这种激活可能在调节摄食中起作用。
