Andrawus Elias, Veildbaum Gizi, Zemel Esther, Leibu Rina, Perlman Ido, Shehadeh Naim
The Ruth and Bruce Rappaport Faculty of Medicine, Technion, Haifa, Israel.
Rappaport Family Institute for Research in the Medical Sciences, Haifa, Israel.
Transl Vis Sci Technol. 2017 Jul 3;6(4):1. doi: 10.1167/tvst.6.4.1. eCollection 2017 Jul.
The purpose of the study was to assess potential interactions of light exposure and hyperglycemia upon ocular complications in diabetic rats.
Streptozotocin-induced (STZ-induced) diabetic rats ( = 39) and non-diabetic rats ( = 9) were distributed into eight groups according to the irradiance and color of the light phase during the 12/12-hour light/dark regime. Follow-up lasted 90 days and included assessment of cataract development and electroretinogram (ERG) recordings. Stress to the retina was also assessed by glial fibrillary acidic protein immunocytochemistry.
Cataract development was fast in diabetic rats that were exposed to unattenuated white light or to bright colored lights during the light phase. Diabetic rats that were kept under attenuated brown or yellow light during the light phase exhibited slower rate of cataract development. Electroretinogram responses indicated very severe retinal damage in diabetic rats kept under bright colored lights in the blue-yellow range or bright white light during the light phase. Electroretinogram damage was milder in rats kept under bright red light or attenuated yellow or brown light during the light phase. Glial fibrillary acidic protein expression in retinal Müller cells was consistent with ERG assessment of retinal damage.
Attenuating white light and filtering out short wavelengths have a protective effect on the eyes of diabetic rats as evident by slower rate of cataract formation and a smaller degree of retinal damage.
Our findings suggest that special glasses attenuating light exposure and filtering out short wavelengths (400-530 nm) may be beneficial for diabetic patients.
本研究旨在评估光照和高血糖对糖尿病大鼠眼部并发症的潜在相互作用。
将链脲佐菌素诱导（STZ诱导）的糖尿病大鼠（n = 39）和非糖尿病大鼠（n = 9）根据12/12小时光照/黑暗周期中光照阶段的辐照度和颜色分为八组。随访持续90天，包括评估白内障发展情况和视网膜电图（ERG）记录。还通过胶质纤维酸性蛋白免疫细胞化学评估视网膜应激。
在光照阶段暴露于未衰减白光或明亮彩色光的糖尿病大鼠中，白内障发展迅速。在光照阶段处于衰减棕色或黄色光下的糖尿病大鼠白内障发展速率较慢。视网膜电图反应表明，在光照阶段处于蓝黄色范围内的明亮彩色光或明亮白光下的糖尿病大鼠存在非常严重的视网膜损伤。在光照阶段处于明亮红光或衰减黄色或棕色光下的大鼠视网膜电图损伤较轻。视网膜Müller细胞中胶质纤维酸性蛋白的表达与视网膜损伤的ERG评估结果一致。
减弱白光并滤除短波长光对糖尿病大鼠的眼睛具有保护作用，这表现为白内障形成速率较慢和视网膜损伤程度较小。
我们的研究结果表明，能减弱光照并滤除短波长（４００－５３０ｎｍ）的特殊眼镜可能对糖尿病患者有益。
