Orhon Idil, Reggiori Fulvio
Department of Cell Biology, University of Groningen, University Medical Center Groningen, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands.
Cells. 2017 Jul 7;6(3):20. doi: 10.3390/cells6030020.
The vast number of implications of autophagy in multiple areas of life sciences and medicine has attracted the interest of numerous scientists that aim to unveil the role of this process in specific physiological and pathological contexts. Cell cultures are one of the most frequently used experimental setup for the investigation of autophagy. As a result, it is essential to assess this highly regulated molecular pathway with efficient and reliable methods. Each method has its own advantages and disadvantages. Here, we present a review summarizing the most established assays used to monitor autophagy induction and progression in cell cultures, in order to guide researchers in the selection of the most optimal solution for their experimental setup and design.
自噬在生命科学和医学多个领域具有广泛影响，吸引了众多科学家的关注，他们旨在揭示这一过程在特定生理和病理背景下的作用。细胞培养是研究自噬最常用的实验设置之一。因此，使用高效可靠的方法评估这一高度调控的分子途径至关重要。每种方法都有其优缺点。在此，我们进行综述，总结用于监测细胞培养中自噬诱导和进展的最成熟检测方法，以指导研究人员为其实验设置和设计选择最优解决方案。
