Dupont N, Leroy C, Hamaï A, Codogno P
Institut Necker-Enfants Malades (INEM), INSERM U1151-CNRS UMR 8253, Université Paris Descartes-Sorbonne Paris Cité, Paris, France.
Institut Necker-Enfants Malades (INEM), INSERM U1151-CNRS UMR 8253, Université Paris Descartes-Sorbonne Paris Cité, Paris, France.
Methods Enzymol. 2017;588:31-40. doi: 10.1016/bs.mie.2016.09.074. Epub 2016 Nov 11.
Macroautophagy, a major lysosomal degradative pathway for cytoplasmic components, is a process that can be stimulated in response to many stressful situations including cancer treatment. The central autophagic organelle is the autophagosome, a double-membrane-bound vacuole that sequesters cytoplasmic material. The ultimate destiny of the autophagosome is fusion with the lysosomal compartment, where cargo, including proteins, is degraded. Here, we report a method to measure the lysosomal degradation of long-lived proteins along the autophagic pathway.
巨自噬是细胞质成分的主要溶酶体降解途径，是一个可因包括癌症治疗在内的许多应激情况而被激活的过程。自噬的核心细胞器是自噬体，它是一种包裹细胞质物质的双膜结合液泡。自噬体的最终命运是与溶酶体区室融合，在那里包括蛋白质在内的货物会被降解。在此，我们报告一种沿着自噬途径测量长寿命蛋白质溶酶体降解的方法。
