Feng Guanping, Ochoa Marien, Maher Jason R, Awad Hani A, Berger Andrew J
The Institute of Optics, University of Rochester, 275 Hutchinson Road, Rochester, New York, 14627.
Department of Biomedical Engineering, University of Rochester, 207 Robert B. Goergen Hall, Rochester, New York, 14627.
J Biophotonics. 2017 Aug;10(8):990-996. doi: 10.1002/jbio.201600317. Epub 2017 May 2.
The development of spatially offset Raman spectroscopy (SORS) has enabled deep, non-invasive chemical characterization of turbid media. Here, we use SORS to measure subcortical bone tissue and depth-resolved biochemical variability in intact, exposed murine bones. We also apply the technique to study a mouse model of the genetic bone disorder osteogenesis imperfecta. The results suggest that SORS is more sensitive to disease-related biochemical differences in subcortical trabecular bone and marrow than conventional Raman measurements.
空间偏移拉曼光谱（SORS）的发展使得对浑浊介质进行深度、非侵入性的化学表征成为可能。在此，我们使用SORS来测量完整暴露的小鼠骨骼中的皮质下骨组织以及深度分辨的生化变异性。我们还应用该技术研究遗传性骨疾病成骨不全症的小鼠模型。结果表明，与传统拉曼测量相比，SORS对皮质下小梁骨和骨髓中与疾病相关的生化差异更为敏感。
