Rovira i Virgili University, Department of Electronic Engineering, IISPV, 43007 Tarragona, Spain.
Spanish Biomedical Research Centre in Diabetes and Associated Metabolic Disorders (CIBERDEM), 28029 Madrid, Spain.
Anal Chem. 2021 Apr 27;93(16):6301-6310. doi: 10.1021/acs.analchem.0c04986. Epub 2021 Apr 15.
Studies on complex biological phenomena often combine two or more imaging techniques to collect high-quality comprehensive data directly , preserving the biological context. Mass spectrometry imaging (MSI) and vibrational spectroscopy imaging (VSI) complement each other in terms of spatial resolution and molecular information. In the past decade, several combinations of such multimodal strategies arose in research fields as diverse as microbiology, cancer, and forensics, overcoming many challenges toward the unification of these techniques. Here we focus on presenting the advantages and challenges of multimodal imaging from the point of view of studying biological samples as well as giving a perspective on the upcoming trends regarding this topic. The latest efforts in the field are discussed, highlighting the purpose of the technique for clinical applications.
研究复杂的生物现象通常需要结合两种或多种成像技术来直接收集高质量的综合数据，同时保持生物背景。质谱成像 (MSI) 和振动光谱成像 (VSI) 在空间分辨率和分子信息方面互补。在过去的十年中，在微生物学、癌症和法医学等多个研究领域出现了几种这样的多模态策略的组合，克服了将这些技术统一的许多挑战。在这里，我们重点从研究生物样本的角度介绍多模态成像的优点和挑战，并对该主题的未来趋势进行展望。讨论了该领域的最新进展，强调了该技术在临床应用中的目的。
