Biomedical Engineering Graduate Program, University of Calgary, Calgary, AB, Canada.
Faculty of Kinesiology, University of Calgary, Calgary, AB, Canada.
J Biomed Mater Res B Appl Biomater. 2018 Apr;106(3):1329-1338. doi: 10.1002/jbm.b.33895. Epub 2017 Jul 7.
Clinical data show that in vitro contact lens friction is related to in vivo comfort. Solutions of biological lubricants hyaluronan (HA) and proteoglycan 4 (PRG4, also known as lubricin) reduce friction at a cornea-polydimethylsiloxane (PDMS) interface. The purpose of this study was to (1) determine if PRG4 can sorb to and lubricate model contact lens materials and (2) assess the boundary lubricating ability of PRG4 and HA compared to saline on model contact lens materials. PRG4 was obtained from bovine cartilage culture and suspended in saline at 300 µg/mL. N,N-Dimethylacrylamidetris (trimethylsiloxy) silane, (DMAA/TRIS) and methacryloxypropyltris (trimethylsiloxy) silane (pHEMA/TRIS) silicone hydrogels were prepared. A previously described in vitro eyelid-hydrogel and cornea-hydrogel biomechanical friction test was used to determine boundary lubricant effect. PRG4 sorption to the hydrogels was assessed using a soak-rinse protocol and western blotting. PRG4 effectively lubricated both silicone hydrogel materials and HA effectively lubricated pHEMA/TRIS, as indicated by a statistically significant reduction in friction compared to the saline control lubricant. An HA and PRG4 combination showed a synergistic effect for pHEMA/TRIS and effectively lubricated DMAA/TRIS. Biological boundary lubricants HA and PRG4 were shown to effectively lubricate silicone hydrogels when in solution. Additionally, HA and PRG4 showed synergistic lubrication for pHEMA/TRIS. The purpose of this study was not to replicate the friction coefficients of contact lenses, but rather to investigate lubricant-surface interactions for common contact lens constituents. These findings contribute to the potential development of biomolecule based lubricant drops for contact lens wearers. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1329-1338, 2018.
临床数据表明，体外隐形眼镜摩擦与体内舒适度有关。生物润滑剂透明质酸（HA）和蛋白聚糖 4（PRG4，也称为润滑素）的溶液可降低角膜-聚二甲基硅氧烷（PDMS）界面的摩擦。本研究的目的是：（1）确定 PRG4 是否可以吸附并润滑模型隐形眼镜材料，以及（2）评估 PRG4 和 HA 与生理盐水相比对模型隐形眼镜材料的边界润滑能力。PRG4 从牛软骨培养物中获得，并悬浮在 300μg/mL 的盐水中。N，N-二甲基丙烯酰胺三（三甲硅氧基）硅烷（DMAA/TRIS）和甲基丙烯酰氧基丙基三（三甲硅氧基）硅烷（pHEMA/TRIS）硅水凝胶被制备。先前描述的体外眼睑-水凝胶和角膜-水凝胶生物力学摩擦试验用于确定边界润滑剂的效果。通过浸泡-冲洗方案和 Western blot 评估 PRG4 对水凝胶的吸附。与盐水对照润滑剂相比，PRG4 有效地润滑了两种硅水凝胶材料，HA 有效地润滑了 pHEMA/TRIS，表明摩擦明显降低。HA 和 PRG4 的组合对 pHEMA/TRIS 显示出协同作用，并有效地润滑 DMAA/TRIS。生物边界润滑剂 HA 和 PRG4 在溶液中有效地润滑硅水凝胶。此外，HA 和 PRG4 对 pHEMA/TRIS 显示出协同润滑作用。本研究的目的不是复制隐形眼镜的摩擦系数，而是研究常见隐形眼镜成分的润滑剂-表面相互作用。这些发现有助于基于生物分子的隐形眼镜佩戴者的润滑剂滴的潜在开发。©2017 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater，106B：1329-1338，2018。
