Heinecke Nayely, Tórtora Jorge, Martínez Humberto A, González-Fernández Víctor D, Ramírez Hugo
Facultad de Estudios Superiores Cuautitlán, Veterinary Medicine, Virology, Genetics and Molecular Biology Laboratory, Universidad Nacional Autónoma de México, Campus 4. Km 2.5 Carretera Cuautitlán-Teoloyucan, San Sebastián Xhala, Cuautitlán Izcalli, Estado de México, CP.54714, México.
Arch Virol. 2017 Oct;162(10):3191-3196. doi: 10.1007/s00705-017-3477-z. Epub 2017 Jul 8.
Bovine leukemia virus (BLV) was detected and genotyped in a population of 201 dairy cattle from central Mexico. Using a commercial indirect enzyme-linked immunosorbent assay (iELISA) kit, 118 polymerase chain reaction (PCR)-positive and BLV antibody-positive samples were identified; the concordance between tests was substantial. A phylogenetic study of 27 partial sequences of the env gene gp30 was performed. Four mutations were detected involving the PXXP motif in the cytoplasmic domain of the transmembrane protein. This study provided evidence of the efficacy of PCR for the detection of BLV and demonstrated the presence of genotype 1 BLV in Mexico.
在来自墨西哥中部的201头奶牛群体中检测并对牛白血病病毒（BLV）进行了基因分型。使用商用间接酶联免疫吸附测定（iELISA）试剂盒，鉴定出118份聚合酶链反应（PCR）阳性且BLV抗体阳性的样本；检测之间的一致性很高。对env基因gp30的27个部分序列进行了系统发育研究。检测到四个突变，涉及跨膜蛋白胞质结构域中的PXXP基序。本研究提供了PCR检测BLV有效性的证据，并证明了墨西哥存在1型BLV。
