柳叶菜提取物对乙酰氨基酚诱导的体内外肝毒性的保护作用。
Hepatoprotective effects of ethanol extracts from Folium Syringae against acetaminophen-induced hepatotoxicity in vitro and in vivo.
机构信息
Basic Veterinary Department, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
Basic Veterinary Department, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
出版信息
J Chin Med Assoc. 2017 Oct;80(10):623-629. doi: 10.1016/j.jcma.2017.03.007. Epub 2017 Jul 6.
BACKGROUND
The leaves of Folium Syringae (FS) have been long used as a traditional Chinese folk medicine for their anti-inflammatory effect, utilized as an antibacterial and antiviral treatment. The purpose of this study was to investigate the potential hepatoprotective effects of FS on acetaminophen-induced hepatic injury in primary hepatocytes and mice.
METHODS
Hepatocytes obtained by the inverse perfusion method were divided randomly into five groups. Prior to acetaminophen exposure, 3 different doses of FS ethanol extracts were given to hepatocytes and mice, respectively. Thereafter, transaminases, glutathione S-transferase A1 (GSTA1) and some hepatic indices were determined.
RESULTS
FS ethanol extracts (200 μg/mL) pretreatment prevented all of the alterations, returning their levels to nearly those levels observed in the control group in vitro. Treatment with FS ethanol extracts (200 mg/kg) significantly reduced the toxicity induced by acetaminophen in vivo, which manifested as a decrease in transaminases, and the hepatoprotective effects of FS were similar to Silymarin (positive group). GSTA1 represented the same change trend as transaminases and hepatic indices, and at a dose of 100 μg/mL FS ethanol extracts in vitro and 100 mg/kg in vivo, GSTA1 content changed significantly (p < 0.01), but transaminases were insignificant (p > 0.05).
CONCLUSION
The results of our investigation suggested that FS ethanol extracts possess significant protective effects against hepatotoxicity induced by acetaminophen both in vitro and in vivo. In addition, GSTA1 could be used as an indicator assessing the extents of hepatic injury, which is more sensitive than transaminases.
背景
柳叶已被长期用作传统中药，具有抗炎作用，可用作抗菌和抗病毒治疗。本研究旨在探讨 FS 叶对乙酰氨基酚诱导的原代肝细胞和小鼠肝损伤的潜在保护作用。
方法
采用逆行灌注法获取肝细胞，随机分为五组。在暴露于对乙酰氨基酚之前，分别给予肝细胞和小鼠三种不同剂量的 FS 乙醇提取物。然后，测定转氨酶、谷胱甘肽 S-转移酶 A1（GSTA1）和一些肝指标。
结果
FS 乙醇提取物（200μg/mL）预处理可防止所有变化，使其水平几乎恢复到体外对照组的水平。FS 乙醇提取物（200mg/kg）治疗可显著降低体内对乙酰氨基酚引起的毒性，表现为转氨酶降低，FS 的肝保护作用与水飞蓟素（阳性组）相似。GSTA1 与转氨酶和肝指标呈相同的变化趋势，体外 FS 乙醇提取物 100μg/mL 和体内 100mg/kg 时 GSTA1 含量变化显著（p<0.01），但转氨酶无显著差异（p>0.05）。
结论
本研究结果表明，FS 乙醇提取物对体内外乙酰氨基酚诱导的肝毒性具有显著的保护作用。此外，GSTA1 可作为评估肝损伤程度的指标，比转氨酶更敏感。
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