Zhang Xiong, Yang Rui, Hu Bei-Lei, Lu Pengcheng, Zhou Li-Li, He Zhi-Yong, Wu Hong-Mei, Zhu Jian-Hong
Department of Geriatrics and Neurology, the Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, WenzhouChina.
Department of Preventive Medicine, Wenzhou Medical University, WenzhouChina.
Front Cell Neurosci. 2017 Jun 23;11:170. doi: 10.3389/fncel.2017.00170. eCollection 2017.
Aberrant expression of microRNA (miRNA) in tissues may lead to altered level in circulation. Considerable evidence has suggested that miRNA deregulation is involved in the pathogenesis of Parkinson's disease (PD). In this study, we screened a set of PD-associated miRNAs and aimed to identify differentially expressed miRNAs in plasma of PD patients and to evaluate their potentiality to serve as PD biomarkers. A total of 95 subjects consisting of 46 sporadic PD cases and 49 controls were recruited. Plasma levels of six miRNAs including miR-433, miR-133b, miR-34b, miR-34c, miR-153, and miR-7 were evaluated using reverse transcribed quantitative PCR, among which we found that miR-34c and miR-7 were below detection limit under our condition. The results showed that levels of circulating miR-433 ( = 0.003) and miR-133b ( = 0.006), but not miR-34b and miR-153, were reduced in PD patients. miR-433 and miR-133b were strongly correlated in both control and PD groups ( = 0.87 and 0.85, respectively). The correlation between miR-34b and miR-153 expressions was significantly reduced ( < 0.05) in the PD group. Although miR-433 and miR-133b were likely to be functionally complimentary as suggested by Pathway and Gene Ontology analyses, these two miRNAs might not be sufficient to predict PD. No correlation was observed between the four miRNAs and age or severity of disease. Collectively, our results demonstrate that circulating miR-433 and miR-133b are significantly altered in PD and may serve as PD biomarkers.
组织中微小RNA（miRNA）的异常表达可能导致循环水平的改变。大量证据表明，miRNA失调与帕金森病（PD）的发病机制有关。在本研究中，我们筛选了一组与PD相关的miRNA，旨在鉴定PD患者血浆中差异表达的miRNA，并评估它们作为PD生物标志物的潜力。共招募了95名受试者，其中包括46例散发性PD病例和49名对照。使用逆转录定量PCR评估了六种miRNA（包括miR-433、miR-133b、miR-34b、miR-34c、miR-153和miR-7）的血浆水平，其中我们发现在我们的条件下miR-34c和miR-7低于检测限。结果显示，PD患者循环miR-433（P = 0.003）和miR-133b（P = 0.006）的水平降低，但miR-34b和miR-153未降低。miR-433和miR-133b在对照组和PD组中均呈强相关（分别为r = 0.87和0.85）。在PD组中，miR-34b和miR-153表达之间的相关性显著降低（P < 0.05）。尽管通路和基因本体分析表明miR-433和miR-133b可能在功能上互补，但这两种miRNA可能不足以预测PD。未观察到这四种miRNA与年龄或疾病严重程度之间的相关性。总体而言，我们的结果表明，循环miR-433和miR-133b在PD中显著改变，可能作为PD生物标志物。
