Jhingran Anupam, Kasahara Shinji, Hohl Tobias M
Infectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, USA.
Immunology Program, Sloan Kettering Institute; Memorial Sloan Kettering Cancer Center, New York, USA.
Bio Protoc. 2016 Sep 20;6(18). doi: 10.21769/BioProtoc.1927.
is a ubiquitous fungal pathogen that forms airborne conidia. The process of restricting conidial germination into hyphae by lung leukocytes is critical in determining infectious outcomes. Tracking the outcome of conidia-host cell encounters is technically challenging and an obstacle to understanding the molecular and cellular basis of antifungal immunity in the lung. Here, we describe a method that utilizes a genetically engineered strain [called FLARE (Jhingran ., 2012; Espinosa ., 2014; Heung ., 2015)] to monitor conidial phagocytosis and killing by leukocytes within the lung environment at single encounter resolution.
是一种形成空气传播分生孢子的无处不在的真菌病原体。肺白细胞将分生孢子萌发限制为菌丝的过程对于确定感染结果至关重要。追踪分生孢子与宿主细胞相遇的结果在技术上具有挑战性，并且是理解肺部抗真菌免疫的分子和细胞基础的障碍。在这里，我们描述了一种利用基因工程菌株[称为FLARE（Jhingran等人，2012年；Espinosa等人，2014年；Heung等人，2015年）]以单次相遇分辨率监测肺部环境中白细胞对分生孢子的吞噬和杀伤的方法。
