Kim Byoung Soo, Kim Hyeonji, Gao Ge, Jang Jinah, Cho Dong-Woo
Department of Mechanical Engineering, Pohang University of Science and Technology, Republic of Korea.
Biofabrication. 2017 Aug 4;9(3):034104. doi: 10.1088/1758-5090/aa7e98.
In tissue engineering, the need for hierarchical assembly of three-dimensional (3D) tissues has become increasingly important, considering that new technology is essential for advanced tissue fabrication. 3D cell printing has emerged as a powerful technology to recapitulate the microenvironment of native tissue, allowing for the precise deposition of multiple cells onto the pre-defined position. Parallel to these technological advances, the search for an appropriate bioink that can provide a suitable microenvironment supporting cellular activities has been in the spotlight. In this respect, the decellularized extracellular matrix (dECM) becomes a popular candidate as a well-qualified source of bioink because of its capability to inherit the intrinsic cues from a native ECM. Yet, few studies have been reported and its potential has been partially understood in the field of 3D cell printing. In this review, our focus is on a dECM as a prospective bioink to facilitate 3D cell printing-based tissue engineering. We begin this review with a brief description of the important role of the ECM. Next, the representative methods of decellularization and conventional applications of a dECM are introduced, followed by the recent achievements in dECM bioinks and their future directions.
在组织工程中，考虑到新技术对于先进组织制造至关重要，三维（3D）组织的分层组装需求变得越来越重要。3D细胞打印已成为一种强大的技术，可重现天然组织的微环境，允许将多种细胞精确沉积到预定义位置。与这些技术进步并行的是，寻找一种能够提供支持细胞活动的合适微环境的生物墨水一直备受关注。在这方面，脱细胞细胞外基质（dECM）因其能够从天然细胞外基质继承内在信号而成为一种受欢迎的生物墨水合格来源。然而，在3D细胞打印领域，相关研究报道较少，其潜力也仅得到部分理解。在本综述中，我们重点关注dECM作为一种前瞻性生物墨水，以促进基于3D细胞打印的组织工程。我们首先简要描述细胞外基质的重要作用。接下来，介绍脱细胞的代表性方法和dECM的传统应用，随后阐述dECM生物墨水的最新进展及其未来方向。
