Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, NC, 27157, USA.
Department of Chemistry, Faculty of Science, Zagazig University, Zagazig, Sharkia, 44519, Egypt.
Adv Healthc Mater. 2019 Apr;8(7):e1800992. doi: 10.1002/adhm.201800992. Epub 2019 Feb 6.
3D bioprinting strategies in tissue engineering aim to fabricate clinically applicable tissue constructs that can replace the damaged or diseased tissues and organs. One of the main prerequisites in 3D bioprinting is finding an appropriate bioink that provides a tissue-specific microenvironment supporting the cellular growth and maturation. In this respect, decellularized extracellular matrix (dECM)-derived hydrogels have been considered as bioinks for the cell-based bioprinting due to their capability to inherit the intrinsic cues from native ECM. Herein, a photo-crosslinkable kidney ECM-derived bioink (KdECMMA) is developed that could provide a kidney-specific microenvironment for renal tissue bioprinting. Porcine whole kidneys are decellularized through a perfusion method, dissolved in an acid solution, and chemically modified by methacrylation. A KdECMMA-based bioink is formulated and evaluated for rheological properties and printability for the printing process. The results show that the bioprinted human kidney cells in the KdECMMA bioink are highly viable and mature with time. Moreover, the bioprinted renal constructs exhibit the structural and functional characteristics of the native renal tissue. The potential of the tissue-specific ECM-derived bioink is demonstrated for cell-based bioprinting that could enhance the cellular maturation and eventually tissue formation.
三维生物打印技术在组织工程中的目标是制造可临床应用的组织构建体，以替代受损或患病的组织和器官。三维生物打印的主要前提之一是找到合适的生物墨水，为细胞生长和成熟提供组织特异性的微环境。在这方面，由于能够继承天然细胞外基质 (ECM) 的固有信号，脱细胞细胞外基质 (dECM) 衍生的水凝胶已被认为是基于细胞的生物打印的生物墨水。本文开发了一种可光交联的肾脏 ECM 衍生生物墨水 (KdECMMA)，可用于肾脏组织的生物打印，为其提供肾脏特异性的微环境。通过灌注法对猪全肾进行脱细胞处理，然后将其溶解在酸溶液中，并通过甲基丙烯酰化进行化学修饰。本文配制了一种基于 KdECMMA 的生物墨水，并对其流变性能和打印性能进行了评估。结果表明，在 KdECMMA 生物墨水中生物打印的人肾细胞具有较高的活力，并随时间逐渐成熟。此外，生物打印的肾脏构建体表现出天然肾脏组织的结构和功能特征。该研究证明了组织特异性 ECM 衍生生物墨水在基于细胞的生物打印中的潜力，它可以增强细胞成熟度，最终促进组织形成。
