Pati Falguni, Cho Dong-Woo
Department of Biomedical Engineering, Indian Institute Technology Hyderabad, Kandi, Sangareddy, 502285, Telangana, India.
Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam ro, Nam-gu, Pohang, Kyungbuk, 790-784, Republic of Korea.
Methods Mol Biol. 2017;1612:381-390. doi: 10.1007/978-1-4939-7021-6_27.
Bioprinting provides an exciting opportunity to print and pattern all the components that make up a tissue-cells and extracellular matrix (ECM) material-in three dimensions (3D) to generate tissue analogues. A large number of materials have been used for making bioinks; however, majority of them cannot represent the complexity of natural ECM and thus are unable to reconstitute the intrinsic cellular morphologies and functions. We present here a method for making of bioink from decellularized extracellular matrices (dECMs) and a protocol for bioprinting of cell-laden constructs with this novel bioink. The dECM bioink is capable of providing an optimized microenvironment that is conducive to the growth of 3D structured tissue. We have prepared bioinks from different tissues, including adipose, cartilage and heart tissues and achieved high cell viability and functionality of the bioprinted tissue structures using our novel bioink.
生物打印提供了一个令人兴奋的机会，可以在三维空间（3D）中打印和构建构成组织的所有成分——细胞和细胞外基质（ECM）材料，以生成组织类似物。大量材料已被用于制造生物墨水；然而，它们中的大多数无法体现天然ECM的复杂性，因此无法重构内在的细胞形态和功能。我们在此展示一种从脱细胞细胞外基质（dECM）制备生物墨水的方法，以及使用这种新型生物墨水对载有细胞的构建体进行生物打印的方案。dECM生物墨水能够提供一个有利于三维结构化组织生长的优化微环境。我们已经从不同组织，包括脂肪、软骨和心脏组织制备了生物墨水，并使用我们的新型生物墨水实现了生物打印组织结构的高细胞活力和功能。
