Ellis Gregory A, Thomas Chris S, Chanana Shaurya, Adnani Navid, Szachowicz Emily, Braun Doug R, Harper Mary Kay, Wyche Thomas P, Bugni Tim S
Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.
Department of Medicinal Chemistry, University of Utah, Salt Lake City, Utah, United States of America.
PLoS One. 2017 Jul 10;12(7):e0176968. doi: 10.1371/journal.pone.0176968. eCollection 2017.
Bacterial communities associated with marine invertebrates such as sponges and ascidians have demonstrated potential as sources of bio-medically relevant small molecules. Metagenomic analysis has shown that many of these invertebrates harbor populations of Actinobacteria, many of which are cultivable. While some populations within invertebrates are transmitted vertically, others are obtained from the environment. We hypothesized that cultivable diversity from sponges living in brackish mangrove habitats have associations with Actinobacterial populations that differ from those found in clear tropical waters. In this study, we analyzed the cultivable Actinobacterial populations from sponges found in these two distinct habitats with the aim of understanding the secondary metabolite potential. Importantly, we wanted to broadly evaluate the potential differences among these groups to guide future Actinobacterial collection strategies for the purposes of drug discovery.
与海绵和海鞘等海洋无脊椎动物相关的细菌群落已显示出作为生物医学相关小分子来源的潜力。宏基因组分析表明，这些无脊椎动物中有许多含有放线菌种群，其中许多是可培养的。虽然无脊椎动物体内的一些种群是垂直传播的，但其他种群是从环境中获得的。我们假设，生活在微咸红树林栖息地的海绵的可培养多样性与放线菌种群的关联不同于在清澈热带水域中发现的放线菌种群。在本研究中，我们分析了在这两个不同栖息地发现的海绵的可培养放线菌种群，目的是了解其次级代谢产物潜力。重要的是，我们希望广泛评估这些群体之间的潜在差异，以指导未来用于药物发现的放线菌收集策略。
