Holderman Chris J, Kaufman Phillip E, Booth Matthew M, Bernier Ulrich R
Entomology and Nematology Department, University of Florida, Gainesville, FL, United States.
Entomology and Nematology Department, University of Florida, Gainesville, FL, United States.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Sep 1;1061-1062:1-4. doi: 10.1016/j.jchromb.2017.06.044. Epub 2017 Jun 27.
Host derived chemical cues are an important aspect of arthropod attraction to potential hosts. Host cues that act over longer distances include CO, heat, and water vapor, while cues such as volatile organic compounds (VOCs) act over closer distances. Domestic dogs are important hosts for disease cycles that include dog heartworm disease vectored by mosquitoes, however the host VOCs utilized by vectors are not well known. Herein we present a novel method that sampled VOCs from a dog host. A Tenax TD stainless steel tube was held near a dog's fur and skin, which collected VOCs that were later desorbed and tentatively identified using a gas chromatograph-mass spectrospectrometer (GC-MS). Background air chemicals were subtracted from the dog sample, resulting in 182 differentiated compounds, a majority of which were identified by ionization fragmentation patterns. Four dogs were sampled and shared 41 of the identified chemicals. VOCs were representative of aliphatics, aromatics, aldehydes, alcohols and carboxylic acids. This chemical characterization method has the potential to identify both individuals and breeds of dogs in addition to other potential uses such as disease diagnosis.
宿主衍生的化学信号是节肢动物吸引潜在宿主的一个重要方面。在较远距离起作用的宿主信号包括一氧化碳、热量和水蒸气，而挥发性有机化合物（VOCs）等信号则在较近距离起作用。家犬是包括由蚊子传播的犬心丝虫病在内的疾病传播循环中的重要宿主，然而病媒所利用的宿主挥发性有机化合物尚不清楚。在此，我们提出了一种从犬宿主中采集挥发性有机化合物的新方法。将一根Tenax TD不锈钢管靠近犬的皮毛和皮肤，收集挥发性有机化合物，随后将其解吸，并使用气相色谱-质谱仪（GC-MS）进行初步鉴定。从犬样本中减去背景空气化学物质，得到182种不同的化合物，其中大部分通过电离碎片模式进行了鉴定。对四只犬进行了采样，它们共有41种已鉴定的化学物质。挥发性有机化合物代表脂肪族、芳香族、醛类、醇类和羧酸类。这种化学表征方法除了可用于疾病诊断等其他潜在用途外，还有识别犬的个体和品种的潜力。
