aPDT 治疗去卵巢大鼠伴系统性尼古丁牙周炎
aPDT for periodontitis treatment in ovariectomized rats under systemic nicotine.
机构信息
São Paulo State University (UNESP), Group for the Research and Study of Laser in Dentistry, Department of Surgery and Integrated Clinic, Division of Periodontics, School of Dentistry, Araçatuba, 1193, Jose Bonifácio, 16015-050, Araçatuba, SP, Brazil.
São Paulo State University (UNESP), Group for the Research and Study of Laser in Dentistry, Department of Surgery and Integrated Clinic, Division of Periodontics, School of Dentistry, Araçatuba, 1193, Jose Bonifácio, 16015-050, Araçatuba, SP, Brazil; São Paulo State University (UNESP), Department of Basic Science, School of Dentistry, Araçatuba, 1193, José Bonifácio. 16015-050, Araçatuba, SP, Brazil.
出版信息
Photodiagnosis Photodyn Ther. 2018 Jun;22:70-78. doi: 10.1016/j.pdpdt.2018.02.017. Epub 2018 Feb 23.
BACKGROUND
This study evaluated the antimicrobial photodynamic therapy (aPDT) as an adjunctive therapy to scaling and root planning (SRP) for the treatment of experimental periodontitis (EP) in ovariectomized rats under systemic nicotine.
METHODS
Female ovariectomized rats (n = 180) were divided into two groups: vehicle administration (Veh) and nicotine administration (Nic). Mini-pumps containing either vehicle or nicotine were inserted in the rats 30 days before the induction of EP, which was induced by placing a ligature around the left mandibular first molar. The rats were randomly divided into three treatment subgroups: SRP, SRP plus low-level laser therapy (LLLT), and SRP plus aPDT. aPDT consisted of the application of a phenothiazine photosensitizer followed by LLLT. Ten animals from each group were euthanized at days 7, 15, and 30 after periodontal treatment. The furcation region was evaluated using histological, histometric analyses and immunolabelling for PCNA, TRAP, RANKL, and OPG.
RESULTS
Nicotine administration resulted in greater bone loss (BL). aPDT resulted in lower BL compared to SRP. aPDT showed higher quantities of PCNA-positive cells compared to SRP, regardless of the nicotine status. aPDT resulted in less recruitment of osteoclasts and lower RANKL immunolabelling compared to LLLT and SRP.
CONCLUSION
aPDT was effective in animals treated with nicotine.
背景
本研究评估了光动力抗菌疗法（aPDT）作为辅助治疗，用于治疗去卵巢大鼠伴系统性尼古丁的实验性牙周炎（EP），并与龈下刮治和根面平整（SRP）进行对比。
方法
雌性去卵巢大鼠（n=180）分为两组：对照组（Veh）和尼古丁组（Nic）。在诱导 EP 之前 30 天，将含有对照物或尼古丁的微型泵插入大鼠体内，通过结扎左下颌第一磨牙诱导 EP。大鼠随机分为三组：SRP、SRP 联合低水平激光治疗（LLLT）和 SRP 联合 aPDT。aPDT 包括应用苯并噻嗪光敏剂，然后进行 LLLT。每组各有 10 只动物在牙周治疗后第 7、15 和 30 天处死。使用组织学、组织计量分析和 PCNA、TRAP、RANKL 和 OPG 的免疫标记物评估分叉区。
结果
尼古丁组导致更大的骨丧失（BL）。与 SRP 相比，aPDT 导致更低的 BL。与 SRP 相比，aPDT 显示出更多的 PCNA 阳性细胞，无论尼古丁状态如何。与 LLLT 和 SRP 相比，aPDT 导致破骨细胞募集减少和 RANKL 免疫标记物减少。
结论
aPDT 在接受尼古丁治疗的动物中有效。
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