BRCA1表达在结肠癌细胞对长春新碱的耐药性中起作用。
BRCA1 expression serves a role in vincristine resistance in colon cancer cells.
作者信息
Xu Zhongjie, Zhang Lirong
机构信息
School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China.
Department of Life Sciences and Technology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
出版信息
Oncol Lett. 2017 Jul;14(1):345-348. doi: 10.3892/ol.2017.6149. Epub 2017 May 10.
The present study aimed to investigate the association between breast cancer susceptibility gene 1 (BRCA1) expression and drug resistance in colon cancer, with the specific aim of elucidating the underlying molecular mechanisms of vincristine (VCR) resistance in tumor cells. The HCT-8 human colon cancer cell line was used to establish the VCR-resistant HCT-8/V line by gradually increasing the concentration of VCR during cell culture. The relative mRNA and protein expression levels of BRCA1 in these colon cancer cell lines was assessed by reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) analysis and western blotting, respectively. Resistance to VCR was established in the HCT-8/V colon cancer cells, and RT-qPCR and western blot analysis revealed the expression of BRCA1 to be significantly higher in the VCR-resistant cells compared with their drug-sensitive counterparts (P<0.05). The decreased BRCA1 expression in these VCR-resistant cells may be associated with the drug resistance frequently observed in colon cancer.
本研究旨在探讨乳腺癌易感基因1（BRCA1）表达与结肠癌耐药性之间的关联，具体目的是阐明肿瘤细胞中长春新碱（VCR）耐药的潜在分子机制。在细胞培养过程中，通过逐渐增加VCR浓度，使用HCT-8人结肠癌细胞系建立了VCR耐药的HCT-8/V细胞系。分别通过逆转录定量聚合酶链反应（RT-qPCR）分析和蛋白质印迹法评估这些结肠癌细胞系中BRCA1的相对mRNA和蛋白质表达水平。在HCT-8/V结肠癌细胞中建立了对VCR的耐药性，RT-qPCR和蛋白质印迹分析显示，与药物敏感的对应细胞相比，VCR耐药细胞中BRCA1的表达显著更高（P<0.05）。这些VCR耐药细胞中BRCA1表达的降低可能与结肠癌中常见的耐药性有关。
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