（原文中“Downregulation of ”不完整，缺少具体内容，无法准确完整翻译，仅按现有内容翻译为） 的下调与结肠癌细胞对长春新碱的耐药性有关。
Downregulation of is related to vincristine resistance in colon cancer cells.
作者信息
Han Zhongmin, Huang Hemei, Zhang Tao
机构信息
Department of Medical Technology, Zhengzhou Railway Vocational and Technical College, Zhengzhou, Henan 450052, P.R. China.
出版信息
J Cancer Res Ther. 2019 Jan-Mar;15(1):38-41. doi: 10.4103/0973-1482.192766.
OBJECTIVE
This study was aimed to investigate the relationship between the expression of drebrin (DBN1) gene and resistance in colon cancer to reveal the mechanism of tumor drug resistance and provide a basis for the reversal of this drug resistance in tumor cells.
MATERIALS AND METHODS
The human colon carcinoma cell line HCT-8 was used, and vincristine (VCR)-resistant colon cancer cell line HCT-8/V was established by gradually increasing the concentration of VCR. Polymerase chain reaction (PCR) primers were designed for DBN1 gene. The DBN1 differential expression in colon cancer sensitive and resistant cell lines was detected by fluorescence quantitative PCR. Western blot analysis was used to study DBN1 expression in the resistant cells further.
RESULTS
VCR resistance of HCT-8/V cell line was established. Quantitative PCR and Western blot results showed that DBN1 expression in the resistant cell line was significantly lower, the difference being statistically significant (P < 0.05).
CONCLUSION
Low DBN1 gene expression may be associated with colon cancer cell resistance to VCR.
目的
本研究旨在探讨脑发育蛋白（DBN1）基因表达与结肠癌耐药性之间的关系，以揭示肿瘤耐药机制，并为逆转肿瘤细胞的这种耐药性提供依据。
材料与方法
采用人结肠癌细胞系HCT-8，通过逐步增加长春新碱（VCR）浓度建立VCR耐药结肠癌细胞系HCT-8/V。设计针对DBN1基因的聚合酶链反应（PCR）引物。通过荧光定量PCR检测结肠癌敏感和耐药细胞系中DBN1的差异表达。采用蛋白质免疫印迹分析进一步研究耐药细胞中DBN1的表达。
结果
建立了HCT-8/V细胞系的VCR耐药性。定量PCR和蛋白质免疫印迹结果显示，耐药细胞系中DBN1表达明显降低，差异有统计学意义（P < 0.05）。
结论
DBN1基因低表达可能与结肠癌细胞对VCR耐药有关。
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