Yang Meiliu, Bai Lu, Yu Wu, Sun Xueling, Xu Gang, Guan Ruhua, Yang Ying, Qiu Mingyue, Zhang Yazhong, Tian Jinli, Fang Hui
Department of Medical Experimental Center, North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China.
Department of Surgery, Qianan Yejituo Hospital, Qianan, Hebei 064400, P.R. China.
Oncol Lett. 2017 Jul;14(1):411-415. doi: 10.3892/ol.2017.6101. Epub 2017 Apr 28.
The present study was designed to assess the protein expression of the autophagy-associated genes, Beclin-1 and microtubule-associated protein 1 light chain 3 (LC3)-II, as well as the association with clinicopathological features in papillary thyroid carcinoma (PTC). A total of 50 subjects were recruited, including 50 human PTC samples and paired adjacent noncancerous tissue samples. The protein expression of Beclin-1 and LC3-II was analyzed using immunohistochemistry and western blotting. Beclin-1 and LC3-II expression in PTC tissues significantly reduced compared with normal tissues (P<0.05). Expression of Beclin-1 and LC3-II was associated with lymph node metastasis of PTC (P<0.05), but had no association with age, gender, tumor size, tumor number and Tumor-Node-Metastasis stage (P>0.05). Expression of Beclin-1 and LC3-II were positively correlated (r=0.327;P=0.020) in PTC. In conclusion, the activity of autophagy was declined in PTC; this decrease in autophagic capacity may be associated with tumorigenesis and the development of PTC.
本研究旨在评估自噬相关基因Beclin-1和微管相关蛋白1轻链3（LC3）-II的蛋白表达，以及其与甲状腺乳头状癌（PTC）临床病理特征的相关性。共招募了50名受试者，包括50份人类PTC样本和配对的相邻非癌组织样本。采用免疫组织化学和蛋白质印迹法分析Beclin-1和LC3-II的蛋白表达。与正常组织相比，PTC组织中Beclin-1和LC3-II的表达显著降低（P< 0.05）。Beclin-1和LC3-II的表达与PTC的淋巴结转移相关（P< 0.05），但与年龄、性别、肿瘤大小、肿瘤数量和肿瘤-淋巴结-转移分期无关（P> 0.05）。在PTC中，Beclin-1和LC3-II的表达呈正相关（r=0.327；P=0.020）。总之，PTC中自噬活性下降；自噬能力的这种降低可能与PTC的肿瘤发生和发展有关。
