• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

不同部位及不同传代次数提取的犬脂肪组织来源间充质干细胞特性比较。

Comparison of the characteristics of canine adipose tissue-derived mesenchymal stem cells extracted from different sites and at different passage numbers.

作者信息

Yaneselli Kevin M, Kuhl Cristiana P, Terraciano Paula B, de Oliveira Fernanda S, Pizzato Sabrina B, Pazza Kamila, Magrisso Alessandra B, Torman Vanessa, Rial Analía, Moreno María, Llambí Silvia, Cirne-Lima Elizabeth, Maisonnave Jacqueline

机构信息

Laboratory of Immunology, Department of Microbiological Science, Faculty of Veterinary, Universidad de la República, Montevideo 11600, Uruguay.

Laboratory of Embryology and Cellular Differentiation, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS 90035-903, Brazil.

出版信息

J Vet Sci. 2018 Jan 31;19(1):13-20. doi: 10.4142/jvs.2018.19.1.13.

DOI:10.4142/jvs.2018.19.1.13
PMID:28693305
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5799390/
Abstract

Mesenchymal stem cells (MSCs) have desirable characteristics for use in therapy in animal models and veterinary medicine, due to their capacity of inducing tissue regeneration and immunomodulation. The objective of this study was to evaluate the differences between canine adipose tissue-derived MSCs (AD-MSCs) extracted from subcutaneous (Sc) and visceral (Vs) sites. Surface antigenic markers, differentiation, and mineralized matrix quantification of AD-MSCs at different passages (P, P, and P) were studied. Immunophenotypic analysis showed that AD-MSCs from both sites were CD44+, CD90+, and CD45-. Moreover, they were able, , to differentiate into fat, cartilage, and bone. Sc-AD-MSCs preserve multipotentiality up to P, but Vs-AD-MSCs only tri-differentiated up to P. In addition, compared to Vs-AD-MSCs, Sc-AD-MSCs had greater capacity for mineralized matrix synthesis. In conclusion, Sc-AD-MSCs have advantages over Vs-AD-MSCs, as Sc AD-MSCs preserve multipotentiality during a greater number of passages, have more osteogenic potential, and require less invasive extraction.

摘要

间充质干细胞（MSCs）因其具有诱导组织再生和免疫调节的能力，在动物模型治疗和兽医学中具有理想的应用特性。本研究的目的是评估从皮下（Sc）和内脏（Vs）部位提取的犬脂肪组织来源的间充质干细胞（AD-MSCs）之间的差异。研究了不同传代（P1、P2和P3）的AD-MSCs的表面抗原标志物、分化情况和矿化基质定量。免疫表型分析表明，来自两个部位的AD-MSCs均为CD44+、CD90+和CD45-。此外，它们能够分化为脂肪、软骨和骨。Sc-AD-MSCs在P3之前保持多能性，但Vs-AD-MSCs仅在P2之前进行三向分化。此外，与Vs-AD-MSCs相比，Sc-AD-MSCs具有更强的矿化基质合成能力。总之，Sc-AD-MSCs比Vs-AD-MSCs具有优势，因为Sc-AD-MSCs在更多传代过程中保持多能性，具有更强的成骨潜力，且提取时所需的侵入性更小。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce39/5799390/039411850ad7/jvs-19-13-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce39/5799390/684b61b26117/jvs-19-13-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce39/5799390/7158d2ca8c1b/jvs-19-13-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce39/5799390/3d48330eb812/jvs-19-13-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce39/5799390/0c360de8cd6b/jvs-19-13-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce39/5799390/039411850ad7/jvs-19-13-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce39/5799390/684b61b26117/jvs-19-13-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce39/5799390/7158d2ca8c1b/jvs-19-13-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce39/5799390/3d48330eb812/jvs-19-13-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce39/5799390/0c360de8cd6b/jvs-19-13-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce39/5799390/039411850ad7/jvs-19-13-g005.jpg

相似文献

1
Comparison of the characteristics of canine adipose tissue-derived mesenchymal stem cells extracted from different sites and at different passage numbers.不同部位及不同传代次数提取的犬脂肪组织来源间充质干细胞特性比较。
J Vet Sci. 2018 Jan 31;19(1):13-20. doi: 10.4142/jvs.2018.19.1.13.
2
Sequential sub-passage decreases the differentiation potential of canine adipose-derived mesenchymal stem cells.连续传代降低了犬脂肪来源间充质干细胞的分化潜能。
Res Vet Sci. 2014 Apr;96(2):267-75. doi: 10.1016/j.rvsc.2013.12.011. Epub 2013 Dec 30.
3
Canine mesenchymal stem cells (MSCs): characterization in relation to donor age and adipose tissue-harvesting site.犬骨髓间充质干细胞（MSCs）：与供体年龄和脂肪组织采集部位相关的特征。
Cell Biol Int. 2013 Aug;37(8):789-98. doi: 10.1002/cbin.10090. Epub 2013 Apr 11.
4
A fat option for the pig: hepatocytic differentiated mesenchymal stem cells for translational research.猪的脂肪选择：肝源性分化间充质干细胞用于转化研究。
Exp Cell Res. 2014 Feb 15;321(2):267-75. doi: 10.1016/j.yexcr.2013.10.018. Epub 2013 Nov 4.
5
Isolation, characterization, and in vitro proliferation of canine mesenchymal stem cells derived from bone marrow, adipose tissue, muscle, and periosteum.源自骨髓、脂肪组织、肌肉和骨膜的犬间充质干细胞的分离、特性鉴定及体外增殖
Am J Vet Res. 2012 Aug;73(8):1305-17. doi: 10.2460/ajvr.73.8.1305.
6
Immunophenotype and gene expression profile of mesenchymal stem cells derived from canine adipose tissue and bone marrow.犬脂肪组织和骨髓来源间充质干细胞的免疫表型及基因表达谱
Vet Immunol Immunopathol. 2014 Sep 15;161(1-2):21-31. doi: 10.1016/j.vetimm.2014.06.002. Epub 2014 Jul 1.
7
Characterization of Canine Adipose-Derived Mesenchymal Stromal/Stem Cells in Serum-Free Medium.犬脂肪间充质基质/干细胞在无血清培养基中的特征。
Tissue Eng Part C Methods. 2018 Jul;24(7):399-411. doi: 10.1089/ten.TEC.2017.0409. Epub 2018 Jun 20.
8
Comparative analysis of human mesenchymal stem cells from bone marrow and adipose tissue under xeno-free conditions for cell therapy.无血清条件下用于细胞治疗的人骨髓间充质干细胞与脂肪组织间充质干细胞的比较分析
Stem Cell Res Ther. 2015 Apr 13;6(1):55. doi: 10.1186/s13287-015-0066-5.
9
Extensive characterization of feline intra-abdominal adipose-derived mesenchymal stem cells.猫腹腔脂肪来源间充质干细胞的广泛表征
J Vet Sci. 2017 Sep 30;18(3):299-306. doi: 10.4142/jvs.2017.18.3.299.
10
Viability, yield and expansion capability of feline MSCs obtained from subcutaneous and reproductive organ adipose depots.从皮下和生殖器官脂肪组织中分离的猫间充质干细胞的活力、产量和扩增能力。
BMC Vet Res. 2021 Jul 15;17(1):244. doi: 10.1186/s12917-021-02948-0.

引用本文的文献

1
Exercise-Activated Mesenchymal Stem Cells: A Translational Strategy for Age-Related Sarcopenia Management.运动激活的间充质干细胞：一种用于管理与年龄相关的肌肉减少症的转化策略。
Stem Cell Rev Rep. 2025 Sep 3. doi: 10.1007/s12015-025-10969-7.
2
Mesenchymal stromal cell isolation from pond slider () adipose tissue obtained during routine neutering: a model for turtle species.从日常绝育手术中获取的红耳龟脂肪组织中分离间充质基质细胞：一种龟类模型。
Front Vet Sci. 2025 Mar 19;12:1546091. doi: 10.3389/fvets.2025.1546091. eCollection 2025.
3
Morphologic, Proliferative, and Cytogenetic Changes during In Vitro Propagation of Cat Adipose Tissue-Derived Mesenchymal Stromal/Stem Cells.

本文引用的文献

1
In Vitro Differentiation of First Trimester Human Umbilical Cord Perivascular Cells into Contracting Cardiomyocyte-Like Cells.孕早期人脐带血管周围细胞体外分化为收缩性心肌样细胞
Stem Cells Int. 2016;2016:7513252. doi: 10.1155/2016/7513252. Epub 2016 Mar 30.
2
Cryopreservation of equine mesenchymal stem cells in 95% autologous serum and 5% DMSO does not alter post-thaw growth or morphology in vitro compared to fetal bovine serum or allogeneic serum at 20 or 95% and DMSO at 10 or 5.与含有20%或95%胎牛血清或同种异体血清以及10%或5%二甲基亚砜（DMSO）相比，在95%自体血清和5%DMSO中对马间充质干细胞进行冷冻保存不会改变解冻后体外的生长或形态。
Stem Cell Res Ther. 2015 Nov 26;6:231. doi: 10.1186/s13287-015-0230-y.
3
猫脂肪组织来源的间充质基质/干细胞体外增殖过程中的形态学、增殖和细胞遗传学变化
Animals (Basel). 2024 Aug 20;14(16):2408. doi: 10.3390/ani14162408.
4
Impact of different formulations of platelet lysate on proliferative and immune profile of equine mesenchymal stromal cells.不同配方血小板裂解液对马间充质基质细胞增殖和免疫特征的影响
Front Vet Sci. 2024 Aug 5;11:1410855. doi: 10.3389/fvets.2024.1410855. eCollection 2024.
5
The Omentum-A Forgotten Structure in Veterinary Surgery in Small Animals' Surgery.网膜——小动物外科中兽医外科被遗忘的结构。
Animals (Basel). 2024 Jun 21;14(13):1848. doi: 10.3390/ani14131848.
6
Innervation of adipocytes is limited in mouse perivascular adipose tissue.脂肪细胞对脂肪组织的神经支配有限。
Am J Physiol Heart Circ Physiol. 2024 Jul 1;327(1):H155-H181. doi: 10.1152/ajpheart.00041.2024. Epub 2024 May 24.
7
Impact of multiple isolation procedures on the differentiation potential of adipose derived canine mesenchymal stem cells.多种分离程序对犬脂肪间充质干细胞分化潜能的影响
Am J Stem Cells. 2024 Feb 25;13(1):27-36. doi: 10.62347/LEVZ7282. eCollection 2024.
8
Influence of the Anatomical Site on Adipose Tissue-Derived Stromal Cells' Biological Profile and Osteogenic Potential in Companion Animals.解剖部位对伴侣动物脂肪组织来源基质细胞生物学特性和成骨潜能的影响
Vet Sci. 2023 Nov 24;10(12):673. doi: 10.3390/vetsci10120673.
9
Osteogenic differentiation of adipose-derived canine mesenchymal stem cells seeded in porous calcium-phosphate scaffolds.接种于多孔磷酸钙支架上的犬脂肪来源间充质干细胞的成骨分化
Front Vet Sci. 2023 Jun 2;10:1149413. doi: 10.3389/fvets.2023.1149413. eCollection 2023.
10
Isolation and characterization of feline dental pulp stem cells.猫牙髓干细胞的分离与鉴定。
J Feline Med Surg. 2023 Feb;25(2):1098612X221150625. doi: 10.1177/1098612X221150625.
Cell therapy for chemically induced ovarian failure in mice.
小鼠化学诱导性卵巢功能衰竭的细胞治疗
Stem Cells Int. 2014;2014:720753. doi: 10.1155/2014/720753. Epub 2014 Dec 4.
4
Immunophenotype and gene expression profile of mesenchymal stem cells derived from canine adipose tissue and bone marrow.犬脂肪组织和骨髓来源间充质干细胞的免疫表型及基因表达谱
Vet Immunol Immunopathol. 2014 Sep 15;161(1-2):21-31. doi: 10.1016/j.vetimm.2014.06.002. Epub 2014 Jul 1.
5
Valproic acid promotes differentiation of hepatocyte-like cells from whole human umbilical cord-derived mesenchymal stem cells.丙戊酸促进全人脐带间充质干细胞向肝样细胞分化。
Tissue Cell. 2014 Apr;46(2):127-35. doi: 10.1016/j.tice.2013.12.006. Epub 2013 Dec 31.
6
Sequential sub-passage decreases the differentiation potential of canine adipose-derived mesenchymal stem cells.连续传代降低了犬脂肪来源间充质干细胞的分化潜能。
Res Vet Sci. 2014 Apr;96(2):267-75. doi: 10.1016/j.rvsc.2013.12.011. Epub 2013 Dec 30.
7
Translating stem cell therapies: the role of companion animals in regenerative medicine.翻译干细胞疗法：伴侣动物在再生医学中的作用。
Wound Repair Regen. 2013 May-Jun;21(3):382-94. doi: 10.1111/wrr.12044. Epub 2013 Apr 29.
8
Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT).脂肪组织源基质血管成分的基质细胞和经培养扩增的脂肪组织源基质/干细胞：脂肪治疗与科学国际联合会（IFATS）和国际细胞治疗学会（ISCT）的联合声明。
Cytotherapy. 2013 Jun;15(6):641-8. doi: 10.1016/j.jcyt.2013.02.006. Epub 2013 Apr 6.
9
Canine mesenchymal stem cells (MSCs): characterization in relation to donor age and adipose tissue-harvesting site.犬骨髓间充质干细胞（MSCs）：与供体年龄和脂肪组织采集部位相关的特征。
Cell Biol Int. 2013 Aug;37(8):789-98. doi: 10.1002/cbin.10090. Epub 2013 Apr 11.
10
Comparing the osteogenic potential of canine mesenchymal stem cells derived from adipose tissues, bone marrow, umbilical cord blood, and Wharton's jelly for treating bone defects.比较源自脂肪组织、骨髓、脐带血和华通氏胶的犬间充质干细胞治疗骨缺损的成骨潜力。
J Vet Sci. 2012 Sep;13(3):299-310. doi: 10.4142/jvs.2012.13.3.299.