Merviel Philippe, Cabry Rosalie, Chardon Karen, Haraux Elodie, Scheffler Florence, Mansouri Naima-Belhadri, Devaux Aviva, Chahine Hikmat, Bach Véronique, Copin Henri, Benkhalifa Moncef
Ob/Gyn Department, Regional University hospital, Morvan University, 29200, Brest, France.
ART and Reproductive Biology laboratory, University hospital and school of medicine, Picardie University Jules Verne, CHU Sud, 80054, Amiens, France.
J Ovarian Res. 2017 Jul 10;10(1):42. doi: 10.1186/s13048-017-0335-2.
Oocyte quality is a key limiting factor in female fertility which is primarily reflected in morphological features. Centrally located cytoplasm granulation (CLCG) is one type of cytoplasmic dimorphism exhibited by oocytes that could be linked to pesticide exposure with a significant risk of decreased ICSI outcomes.
This retrospective study included 633 women who were part of an intracytoplasmic spermatozoa injection (ICSI) program between 2009 and 2011. The participants lived in the Picardy region of France and had been exposed to pesticides. The participants were divided in two groups based on prevalence of oocytes with CLCG (LCLCG [n = 83]: low prevalence of oocytes with CLCG under 25%. HCLCG [n = 68]: high prevalence of CLCG over 75%). The embryological and clinical outcomes were analysed for both groups and were calculated using the difference between the two values.
Results for couples with HCLCG compared to LCLCG showed a decrease in embryo cleavage, ongoing pregnancy, and live birth rates (82%, 14%, 13% vs 99%, 32%, 30%, respectively).The early miscarriage rate was increased (47% vs 11%), with an OR of 3.1 (95%CI [2.1-4.1]). Due to high pesticide exposure (over 3000 g/ha), there is a higher risk of a resulting disturbed oocyte cohort with a high prevalence of CLCG over 75%.
The high prevalence of oocytes with CLCG over 75% has a negative effect on embryos and the general ICSI clinical outcomes. Furthermore, a putative association between pesticide exposure and risk of CLCG was identified, justifying the need for further research and a potential need to find alternative assisted reproductive technologies for these couples.
Tabacfertimasc. ID number: ID2011-A00634-37 ; registered 2011/2/8.
卵母细胞质量是女性生育能力的关键限制因素，主要体现在形态特征上。位于中央的细胞质颗粒化（CLCG）是卵母细胞表现出的一种细胞质二态性，可能与接触农药有关，存在显著降低卵胞浆内单精子注射（ICSI）成功率的风险。
这项回顾性研究纳入了2009年至2011年间参与卵胞浆内单精子注射（ICSI）项目的633名女性。参与者居住在法国皮卡第地区，且接触过农药。根据具有CLCG的卵母细胞的患病率将参与者分为两组（LCLCG [n = 83]：具有CLCG的卵母细胞患病率低，低于25%。HCLCG [n = 68]：CLCG患病率高，超过75%）。对两组的胚胎学和临床结局进行分析，并使用两个值之间的差异进行计算。
与LCLCG组相比，HCLCG组夫妇的胚胎分裂率、持续妊娠率和活产率下降（分别为82%、14%、13% 对比99%、32%、30%）。早期流产率增加（47% 对比11%），优势比为3.1（95%置信区间[2.1 - 4.1]）。由于高农药暴露（超过3000克/公顷），出现CLCG患病率超过75%的受干扰卵母细胞群体的风险更高。
CLCG患病率超过75%的卵母细胞对胚胎和总体ICSI临床结局有负面影响。此外，确定了农药暴露与CLCG风险之间的假定关联，证明有必要进行进一步研究，并可能需要为这些夫妇寻找替代辅助生殖技术。
Tabacfertimasc。识别号：ID2011 - A00634 - 37；于2011年2月8日注册。
