Voong Lilien N, Xi Liqun, Wang Ji-Ping, Wang Xiaozhong
Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA.
Department of Statistics, Northwestern University, Evanston, IL 60208, USA.
Trends Genet. 2017 Aug;33(8):495-507. doi: 10.1016/j.tig.2017.05.007. Epub 2017 Jul 7.
Nucleosomes regulate the transcription output of the genome by occluding the underlying DNA sequences from DNA-binding proteins that must act on it. Knowledge of the precise locations of nucleosomes in the genome is thus essential towards understanding how transcription is regulated. Current nucleosome-mapping strategies involve digesting chromatin with nucleases or chemical cleavage followed by high-throughput sequencing. In this review, we compare the traditional micrococcal nuclease (MNase)-based approach with a chemical cleavage strategy, with discussion on the important insights each has uncovered about the role of nucleosomes in shaping transcriptional processes.
核小体通过阻止潜在的DNA序列与必须作用于其上的DNA结合蛋白相互作用,来调节基因组的转录输出。因此,了解核小体在基因组中的精确位置对于理解转录调控机制至关重要。目前的核小体定位策略包括用核酸酶消化染色质或进行化学切割,然后进行高通量测序。在本综述中,我们将基于传统微球菌核酸酶(MNase)的方法与化学切割策略进行比较,并讨论每种方法在揭示核小体在塑造转录过程中的作用方面所获得的重要见解。