真核生物开放核糖体 RNA 基因染色质状态的建立和维持。
Establishment and Maintenance of Open Ribosomal RNA Gene Chromatin States in Eukaryotes.
机构信息
Universität Regensburg, Regensburg Center for Biochemistry (RCB), Lehrstuhl Biochemie III, Regensburg, Germany.
TUM ForTe, Technische Universität München, Munich, Germany.
出版信息
Methods Mol Biol. 2022;2533:25-38. doi: 10.1007/978-1-0716-2501-9_2.
Abstract
In growing eukaryotic cells, nuclear ribosomal (r)RNA synthesis by RNA polymerase (RNAP) I accounts for the vast majority of cellular transcription. This high output is achieved by the presence of multiple copies of rRNA genes in eukaryotic genomes transcribed at a high rate. In contrast to most of the other transcribed genomic loci, actively transcribed rRNA genes are largely devoid of nucleosomes adapting a characteristic "open" chromatin state, whereas a significant fraction of rRNA genes resides in a transcriptionally inactive nucleosomal "closed" chromatin state. Here, we review our current knowledge about the nature of open rRNA gene chromatin and discuss how this state may be established.
摘要
在不断生长的真核细胞中，RNA 聚合酶（RNAP）I 合成核核糖体（r）RNA 占据了细胞转录的绝大部分。这种高产量是通过真核基因组中多个 rRNA 基因的存在实现的，这些基因以高速转录。与大多数其他转录基因组座不同，活跃转录的 rRNA 基因基本上缺乏核小体，适应了一种特征性的“开放”染色质状态，而相当一部分 rRNA 基因位于转录不活跃的核小体“关闭”染色质状态。在这里，我们回顾了我们目前对开放 rRNA 基因染色质性质的了解，并讨论了如何建立这种状态。
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