Meister B, Hökfelt T, Vale W W, Sawchenko P E, Swanson L, Goldstein M
Neuroendocrinology. 1986;42(3):237-47. doi: 10.1159/000124446.
The distribution of growth hormone-releasing factor (GRF) and tyrosine hydroxylase (TH), a marker for dopamine neurons in this region, was analyzed in the mediobasal hypothalamus with indirect immunofluorescence histochemistry and an elution-restaining procedure. TH-like immunoreactivity was present in most GRF-immunoreactive cells in the ventral part of the arcuate nucleus (ventral A12 dopamine cell group). Dopamine cells in the dorsal A12 group and, for example, in the hypothalamic A14 cell group seemed to lack GRF peptide. Partly overlapping GRF- and TH-immunoreactive fibers in the median eminence were observed, indicating possible coexistence of the two compounds also in nerve endings close to portal vessels. These findings suggest that a subpopulation of A12 dopamine neurons produces, stores and releases a GRF-like peptide. Possible interactions of GRF and dopamine in the control of growth hormone secretion are discussed.
采用间接免疫荧光组织化学和洗脱保留法，对下丘脑中间基底部生长激素释放因子（GRF）和酪氨酸羟化酶（TH，该区域多巴胺神经元的标志物）的分布进行了分析。在弓状核腹侧部分（腹侧A12多巴胺细胞群）的大多数GRF免疫反应性细胞中存在TH样免疫反应性。背侧A12组以及例如下丘脑A14细胞群中的多巴胺细胞似乎缺乏GRF肽。在正中隆起处观察到部分重叠的GRF和TH免疫反应性纤维，表明这两种化合物在靠近门静脉的神经末梢中也可能共存。这些发现表明，A12多巴胺神经元的一个亚群产生、储存和释放一种GRF样肽。讨论了GRF和多巴胺在生长激素分泌控制中的可能相互作用。
