在 650mV 以下的氧化还原电位下，无柄嗜酸菌在黄铁矿氧化中的作用有限。
Limited role of sessile acidophiles in pyrite oxidation below redox potential of 650 mV.
机构信息
National Engineering Laboratory for Hydrometallurgical Cleaner Production Technology, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China.
School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing, 100049, China.
出版信息
Sci Rep. 2017 Jul 10;7(1):5032. doi: 10.1038/s41598-017-04420-2.
Pyrite oxidation by mixed mesophilic acidophiles was conducted under conditions of controlled and non-controlled redox potential to investigate the role of sessile microbes in pyrite oxidation. Microbes attached on pyrite surfaces by extracellular polymeric substances (EPS), and their high coverage rate was characterized by scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM) and atomic force microscopy (AFM). The dissolution of pyrite was negligible if the redox potential was controlled below 650 mV (near the rest potential of pyrite), even though the bacteria were highly active and a high coverage rate was observed on pyrite surfaces. However, with un-controlled redox potential the rate of pyrite oxidation increased greatly with an increasing redox potential. This study demonstrates that sessile microbes play a limited role in pyrite oxidation at a redox potential below 650 mV, and highlight the importance of solution redox potential for pyrite oxidation. This has implications for acid mine drainage control and pyrite oxidation control in biometallurgy practice.
采用控制和非控制氧化还原电位的方法研究了混合嗜酸性微生物对黄铁矿的氧化作用，以探讨不活动微生物在黄铁矿氧化中的作用。通过扫描电子显微镜（SEM）、共聚焦激光扫描显微镜（CLSM）和原子力显微镜（AFM）对微生物通过细胞外聚合物质（EPS）附着在黄铁矿表面的情况及其高覆盖率进行了表征。如果氧化还原电位控制在 650 mV 以下（接近黄铁矿的静止电位），黄铁矿的溶解可忽略不计，尽管细菌非常活跃，并且在黄铁矿表面观察到高覆盖率。然而，在非控制氧化还原电位下，随着氧化还原电位的增加，黄铁矿的氧化速率大大增加。这项研究表明，在 650 mV 以下的氧化还原电位下，不活动微生物在黄铁矿氧化中作用有限，并强调了溶液氧化还原电位对黄铁矿氧化的重要性。这对酸性矿山排水控制和生物冶金实践中的黄铁矿氧化控制具有重要意义。
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