豚鼠脑中肾上腺素能神经元的缺失：免疫组织化学与高效液相色谱联用研究
Absence of adrenaline neurons in the guinea pig brain: a combined immunohistochemical and high-performance liquid chromatography study.
作者信息
Cumming P, Von Krosigk M, Reiner P B, McGeer E G, Vincent S R
出版信息
Neurosci Lett. 1986 Jan 16;63(2):125-30. doi: 10.1016/0304-3940(86)90048-0.
The distributions of catecholamine (CA) neurons in the medulla oblongata of the guinea pig and rat were compared using immunohistochemistry with rabbit antisera against the CA synthesizing enzymes, tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (D beta H) and phenylethanolamine-N-methyltransferase (PNMT). TH and D beta H distributions were similar in the two species. In contrast, the central nervous systems of both normal and colchicine-treated guinea pigs failed to demonstrate immunoreactivity for PNMT, the synthetic enzyme for adrenaline. The concentrations of biogenic amines in the hypothalamus and medulla were determined in guinea pig and rat tissues by high-performance liquid chromatography with electrochemical detection. No adrenaline was detected in the guinea pig brain. Thus it appears that guinea pigs lack central neurons capable of synthesizing adrenaline.
利用免疫组织化学方法，使用针对儿茶酚胺（CA）合成酶、酪氨酸羟化酶（TH）、多巴胺-β-羟化酶（DβH）和苯乙醇胺-N-甲基转移酶（PNMT）的兔抗血清，比较了豚鼠和大鼠延髓中CA神经元的分布。TH和DβH的分布在这两个物种中相似。相比之下，正常和秋水仙碱处理的豚鼠中枢神经系统均未显示出对PNMT（肾上腺素合成酶）的免疫反应性。通过高效液相色谱-电化学检测法测定了豚鼠和大鼠组织中下丘脑和延髓中生物胺的浓度。在豚鼠脑中未检测到肾上腺素。因此，豚鼠似乎缺乏能够合成肾上腺素的中枢神经元。
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