Ross C A, Ruggiero D A, Meeley M P, Park D H, Joh T H, Reis D J
Brain Res. 1984 Jul 23;306(1-2):349-53. doi: 10.1016/0006-8993(84)90385-8.
Intraventricular injection of colchicine in rat results in the appearance within hypothalamus of numerous neurons containing the adrenaline-synthesizing enzyme, phenylethanolamine N-methyltransferase, but not the other catecholamine biosynthetic enzymes. Increased PNMT staining in hypothalamus was paralleled by an increase in PNMT activity measured in micropunch preparations. Immunotitration demonstrated that this increase was due to accumulation of specific enzyme protein. The finding that hypothalamic neurons express PNMT without tyrosine hydroxylase suggests that such neurons may produce methylated amines other than adrenaline.
给大鼠脑室内注射秋水仙碱后，下丘脑内会出现许多含有肾上腺素合成酶——苯乙醇胺N-甲基转移酶（PNMT）的神经元，但不含有其他儿茶酚胺生物合成酶。下丘脑内PNMT染色增加的同时，微穿孔制备物中测得的PNMT活性也增加。免疫滴定表明，这种增加是由于特异性酶蛋白的积累。下丘脑神经元表达PNMT而不表达酪氨酸羟化酶这一发现表明，此类神经元可能产生除肾上腺素以外的甲基化胺。
