Benachinmardi Kirtilaxmi K, Ravikumar R, Indiradevi B
Department of Neuromicrobiology, National Institute of Mental Health and Neurosciences, Bengaluru, Karnataka, India.
Department of Neurosurgery, National Institute of Mental Health and Neurosciences, Bengaluru, Karnataka, India.
J Neurosci Rural Pract. 2017 Jul-Sep;8(3):335-341. doi: 10.4103/jnrp.jnrp_22_17.
Biofilms are the source of persistent infections of many pathogenic microbes. They are responsible for nosocomial infection and also associated with many surgical conditions including indwelling medical devices such as ventriculoperitoneal shunt. A significant problem encountered in shunt procedures is obstruction followed by infection, with infection rate ranging from 2% to 27%, often with poor outcome.
This study was conducted in the Department of Neuromicrobiology at a tertiary neuroinstitute for 6 months from July 1 to December 31, 2014. The samples comprised cerebrospinal fluid (CSF) from suspected cases of shunt infections. Laboratory diagnosis of causative agent was established by adopting standard procedures. Then, isolates were evaluated for production of biofilm by tissue culture plate (TCP) method and tube method.
Of the 1642 shunt CSF samples obtained from neurosurgery, 14.79% were culture positive which yielded 254 isolates. About 51.97% were Gram-negative bacilli (GNB), 46.46% were Gram-positive cocci (GPC), and 1.57% were . Among GNB, nonfermenters were the most common (51.52%) followed by (15.9%). Among GPC, coagulase-negative were 88.13%, out of which 43.26% were methicillin-resistant. Other GPC were spp. (4.24%), (5.08%), and spp. (2.54%). Among all isolates, 120 were tested for biofilm production, out of which 57.5% were biofilm producers and 42.5% were nonproducers.
TCP was the better method to detect biofilm. Most of the biofilm producers were resistant pathogens.
生物膜是许多致病微生物持续感染的根源。它们是医院感染的原因，也与许多外科病症相关，包括留置医疗设备如脑室腹腔分流术。分流手术中遇到的一个重大问题是阻塞继之以感染，感染率在2%至27%之间，通常预后不佳。
本研究于2014年7月1日至12月31日在一家三级神经研究所的神经微生物学系进行了6个月。样本包括来自疑似分流感染病例的脑脊液（CSF）。通过采用标准程序对病原体进行实验室诊断。然后，通过组织培养板（TCP）法和试管法评估分离株的生物膜产生情况。
从神经外科获得的1642份分流脑脊液样本中，14.79%培养阳性，共产生254株分离株。约51.97%为革兰氏阴性杆菌（GNB），46.46%为革兰氏阳性球菌（GPC），1.57%为……。在GNB中，非发酵菌最常见（51.52%），其次是……（15.9%）。在GPC中，凝固酶阴性……占88.13%，其中43.26%对甲氧西林耐药。其他GPC为……菌属（4.24%）、……（5.08%）和……菌属（2.54%）。在所有分离株中，对120株进行了生物膜产生检测，其中57.5%为生物膜产生菌，42.5%为非产生菌。
TCP是检测生物膜的更好方法。大多数生物膜产生菌是耐药病原体。
