Guszczyn Tomasz, Surażyński Arkadiusz, Zaręba Ilona, Rysiak Edyta, Popko Janusz, Pałka Jerzy
Department of Pediatric Orthopedics and Traumatology.
Department of Medicinal Chemistry, Medical University of Białystok, Białystok, Poland.
Drug Des Devel Ther. 2017 Jun 22;11:1849-1857. doi: 10.2147/DDDT.S135949. eCollection 2017.
The study was conducted to evaluate the effects of platelet-rich plasma (PRP), supernatant of PRP (SPRP) obtained by centrifugation, and supernatant of activated PRP (SActi-PRP) obtained by Ca solution-treated PRP on collagen biosynthesis, prolidase activity, and β1-integrin signaling in cultured human skin fibroblasts. Incubation of fibroblasts with 5% PRP for 24 h contributed to ~5-fold increase in collagen biosynthesis compared to the control. In the cells treated with 5% of SPRP or SActi-PRP, collagen biosynthesis showed a 3-fold increase of the control. PRP, SPRP, and SActi-PRP stimulated prolidase activity similar to collagen biosynthesis. Collagen biosynthesis and prolidase activity are regulated by β1-integrin receptor signaling. Incubation of fibroblasts with PRP for 24 h contributed to a dose-dependent increase in the expression of β1-integrin receptor, while SActi-PRP increased the process to a much lower extent. SPRP had no effect on the β1-integrin receptor expression. All the studied fractions of blood increased the expression of FAK as well as the expression of phosphorylated MAP-kinases. However, PRP was found to be the most effective stimulator of expression of these particular kinases. These studies suggest that a complex of factors, including growth factors, adhesion molecules, and prolidase contained in PRP, all evoke growth and collagen-promoting activities in human dermal fibroblasts.
本研究旨在评估富血小板血浆（PRP）、通过离心获得的PRP上清液（SPRP）以及通过钙溶液处理PRP获得的活化PRP上清液（SActi-PRP）对培养的人皮肤成纤维细胞中胶原蛋白生物合成、脯氨酰肽酶活性和β1整合素信号传导的影响。与对照组相比，用5% PRP孵育人皮肤成纤维细胞24小时可使胶原蛋白生物合成增加约5倍。在用5%的SPRP或SActi-PRP处理的细胞中，胶原蛋白生物合成显示为对照组的3倍增加。PRP、SPRP和SActi-PRP刺激脯氨酰肽酶活性的方式与胶原蛋白生物合成相似。胶原蛋白生物合成和脯氨酰肽酶活性受β1整合素受体信号传导调节。用PRP孵育人皮肤成纤维细胞24小时导致β1整合素受体表达呈剂量依赖性增加，而SActi-PRP在较低程度上增加了这一过程。SPRP对β1整合素受体表达没有影响。所有研究的血液组分均增加了黏着斑激酶（FAK）的表达以及磷酸化丝裂原活化蛋白激酶（MAP激酶）的表达。然而，发现PRP是这些特定激酶表达的最有效刺激物。这些研究表明，包括PRP中所含生长因子、黏附分子和脯氨酰肽酶在内的多种因素的复合物，均可在人真皮成纤维细胞中引发促进生长和胶原蛋白生成活性。
