Aires Alfredo, Carvalho Rosa
Centre for the Research and Technology for Agro-Environment and Biological Sciences (CITAB), Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal.
Agronomy Department, Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal.
Int J Anal Chem. 2017;2017:5125329. doi: 10.1155/2017/5125329. Epub 2017 Jun 13.
Glucosinolates are a class of sulphur-containing plant compounds with diverse biological properties. They have been found exclusively in the Brassicaceae family plants and studied exhaustively in biosynthetic and application perspectives. The aim of this current study is to present a simple and updated method to quantify indole glucosinolate content in hairy root cultures of Chinese cabbage by HPLC-DAD-UV/Vis. Method validation controls were performed and recovery experiments were assayed. The data was statically treated and compared with published works. The current method allowed a feasible identification of indole glucosinolates and it was possible to identify accurately three indole glucosinolate compounds (glucobrassicin, 4-methoxyglucobrassicin, and 1-methoxyglucobrassicin) in roots of Chinese cabbage. The method demonstrated a good linearity ( > 0.99), a good precision, and selectivity sensitivity. In conclusion, this protocol provides an accessible method to extract and quantify glucosinolates in plant samples. Thus, based on our results, the method is valid and can be extended to other plant or food matrices.
硫代葡萄糖苷是一类具有多种生物学特性的含硫植物化合物。它们仅在十字花科植物中被发现，并已从生物合成和应用角度进行了详尽研究。本研究的目的是提出一种简单且最新的方法，通过高效液相色谱-二极管阵列检测器-紫外/可见分光光度计（HPLC-DAD-UV/Vis）对大白菜毛状根培养物中的吲哚硫代葡萄糖苷含量进行定量。进行了方法验证对照并测定了回收率实验。对数据进行了统计学处理，并与已发表的研究进行了比较。当前方法能够对吲哚硫代葡萄糖苷进行可行的鉴定，并且有可能准确鉴定大白菜根中的三种吲哚硫代葡萄糖苷化合物（硫代葡萄糖苷、4-甲氧基硫代葡萄糖苷和1-甲氧基硫代葡萄糖苷）。该方法显示出良好的线性（>0.99）、良好的精密度和选择性灵敏度。总之，该方案提供了一种提取和定量植物样品中硫代葡萄糖苷的可行方法。因此，根据我们的结果，该方法是有效的，并且可以扩展到其他植物或食品基质。
