Hinkle David M, Kruh-Garcia Nicole A, Kruh Jonathan N, Broccardo Carolyn, Doctor Priyanka, Foster C Stephen
Department of Ophthalmology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA.
Open Ophthalmol J. 2017 Jun 12;11:107-116. doi: 10.2174/1874364101711010107. eCollection 2017.
The aim was to report the aqueous humor moxifloxacin concentration and proteome profile of an individual with bilateral uveitis-like syndrome with pigment dispersion.
Multiple reactions monitoring mass spectrometry quantified the aqueous concentration of moxifloxacin in the affected individual. Shotgun proteomic analysis performed via liquid chromatography tandem mass spectrometry (LC-MS/MS) defined the protein profile in the affected individual and unaffected control samples.
Moxifloxacin was present at higher than expected levels in aqueous humor 18 days following oral administration. One-third of the proteins were identified by significantly lower spectral counts in the aqueous of the individual with moxifloxacin associated uveitis compared to the unaffected control.
Moxifloxacin was detected in aqueous humor 18 days following the completion of oral administration. These results suggest that moxifloxacin toxicity may be responsible for the uveitis-like syndrome with pigment dispersion syndrome induced by moxifloxacin therapy.
报告一名患有双侧色素播散性葡萄膜炎样综合征个体的房水莫西沙星浓度和蛋白质组概况。
采用多反应监测质谱法定量受影响个体房水中莫西沙星的浓度。通过液相色谱串联质谱（LC-MS/MS）进行的鸟枪法蛋白质组分析确定了受影响个体和未受影响对照样本中的蛋白质概况。
口服给药18天后，房水中莫西沙星的含量高于预期水平。与未受影响的对照相比，在莫西沙星相关性葡萄膜炎个体的房水中，三分之一的蛋白质通过显著更低的光谱计数得以鉴定。
口服给药结束18天后在房水中检测到莫西沙星。这些结果表明，莫西沙星毒性可能是莫西沙星治疗引起的色素播散综合征样葡萄膜炎的原因。
