Bonnin Philippe, Pansiot Julien, Paven Elise, Eloi Maxime, Renolleau Sylvain, Baud Olivier, Leger Pierre-Louis, Charriaut-Marlangue Christiane
1 Université Sorbonne Paris Cité, Paris, France.
2 INSERM U965, Université Denis Diderot, Physiologie Clinique - Explorations Fonctionnelles, Hôpital Lariboisière, Paris, France.
J Cereb Blood Flow Metab. 2017 Sep;37(9):3091-3096. doi: 10.1177/0271678X17719614. Epub 2017 Jul 11.
Our objective was to determine whether controlled reflow on one side and/or the other side after bilateral carotid occlusion release could reduce cell death in focal ischemic P14 rats. Arterial blood flow was measured using ultrasonography. Cell death, inflammation and nitrotyrosine were measured using immunofluorescence. When reflow was first induced in the contralateral side, we observed improved outcome markers compared with those when reflow was first induced in the ipsilateral side and/or simultaneous reflow was induced in both sides. Our data suggest that progressive rerouting of arterial flow through the circle of Willis toward the ischemic site reduced cell death.
我们的目标是确定双侧颈动脉闭塞解除后，一侧和/或另一侧的控制性再灌注是否能减少局灶性缺血P14大鼠的细胞死亡。使用超声测量动脉血流。使用免疫荧光法测量细胞死亡、炎症和硝基酪氨酸。当首先在对侧诱导再灌注时，与首先在同侧诱导再灌注和/或两侧同时诱导再灌注相比，我们观察到结局指标有所改善。我们的数据表明，通过 Willis 环向缺血部位逐渐重新引导动脉血流可减少细胞死亡。
