Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110.
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125.
Proc Natl Acad Sci U S A. 2017 Jul 25;114(30):8077-8082. doi: 10.1073/pnas.1621438114. Epub 2017 Jul 10.
A recurrent emerging theme is the targeting of proteins to subcellular microdomains within bacterial cells, particularly to the poles. In most cases, it has been assumed that this localization is critical to the protein's function. uses a type IVB secretion system (T4BSS) to export a large number of protein substrates into the cytoplasm of host cells. Here we show that the export apparatus is localized to the bacterial poles, as is consistent with many T4SS substrates being retained on the phagosomal membrane adjacent to the poles of the bacterium. More significantly, we were able to demonstrate that polar secretion of substrates is critically required for 's alteration of the host endocytic pathway, an activity required for this pathogen's virulence.
一个反复出现的新兴主题是将蛋白质靶向细菌细胞内的亚细胞微区，特别是靶向细菌的两极。在大多数情况下，人们认为这种定位对蛋白质的功能至关重要。利用 IVB 型分泌系统（T4BSS）将大量蛋白质底物输出到宿主细胞的细胞质中。在这里，我们表明， 出口设备定位于细菌的两极，这与许多 T4SS 底物被保留在靠近细菌两极的吞噬体膜上是一致的。更重要的是，我们能够证明底物的极分泌对于 的改变宿主内吞途径是至关重要的，这种活动是这种病原体毒力所必需的。
