Plastic and Reconstructive Surgery Research, University of Manchester, Stopford Building, Oxford Road, Manchester M13 9PT, United Kingdom; Department of Computer Sciences, University of Manchester, Manchester M13 9PL, United Kingdom.
Department of Computer Sciences, University of Manchester, Manchester M13 9PL, United Kingdom.
J Mech Behav Biomed Mater. 2017 Nov;75:75-81. doi: 10.1016/j.jmbbm.2017.06.030. Epub 2017 Jun 27.
Increasing numbers of women undergo breast implantation for cosmetic and reconstructive purposes. Contracture of the fibrous capsule, which encases the implant leads to significant pain and reoperation. Texture, wettability and the cellular reaction to implant surfaces are poorly understood determinants of implant biocompatibility. The aim of this study was to evaluate the in-vitro characteristics of a range of commercial available implants using a macrophage based assay of implant biocompatibility and a quantitative assessment of wettability and texture.
Thirteen commercially available surfaces were subjected to wettability and texture characterisation using scanning and laser confocal microscopy. THP-1 macrophages were cultured on their surfaces and assessed using Integrin αV immunocytochemistry, SEM and RT-PCR for the expression of TNF-Alpha, IL-6, IL-10 and a cytokine array for the production of TNF-alpha, IL-10, IL-1RA and IL1β; important indicators of inflammation and macrophage polarization.
Textured surfaces can be accurately sub-categorized dependent upon roughness and re-entrant features into four main types (macro, micro, meso and nano-textured surfaces). Significant (P < 0.0001) differences in implant hydrophobicity and texture exist. Certain surfaces promoted poor macrophage polarization and an innate potential to foster a proinflammatory response. A subgroup analysis showed that texture had a variable effect on markers of inflammation in these surfaces.
We propose a classification of implant surfaces based on roughness and present a macrophage based assay of breast implant biocompatibility with a quantitative assessment of implant wettability and texture. The breast implant surface-cell interaction is variable and sufficient to alter healing response and capsular contracture fate in-vivo.
越来越多的女性出于美容和重建目的接受乳房植入。纤维囊的收缩，即包裹植入物的纤维囊的收缩，会导致严重的疼痛和再次手术。植入物表面的质地、润湿性和细胞反应是植入物生物相容性的理解程度较差的决定因素。本研究旨在使用基于巨噬细胞的植入物生物相容性测定法和对润湿性和质地的定量评估，评估一系列市售植入物的体外特性。
使用扫描和激光共聚焦显微镜对 13 种市售表面进行润湿性和质地特征分析。将 THP-1 巨噬细胞培养在其表面上，并通过整合素 αV 免疫细胞化学、SEM 和 RT-PCR 评估 TNF-α、IL-6、IL-10 的表达，以及用于 TNF-α、IL-10、IL-1RA 和 IL1β 的细胞因子阵列，这些都是炎症和巨噬细胞极化的重要指标。
纹理表面可以根据粗糙度和再进入特征准确地分为四大类（宏观、微观、中观和纳米纹理表面）。植入物疏水性和质地存在显著差异（P < 0.0001）。某些表面促进了巨噬细胞极化不良和固有促炎反应的潜力。亚组分析表明，纹理对这些表面的炎症标志物有不同的影响。
我们提出了一种基于粗糙度的植入物表面分类方法，并提出了一种基于巨噬细胞的乳房植入物生物相容性测定法，对植入物的润湿性和质地进行定量评估。乳房植入物表面-细胞相互作用是可变的，足以改变体内愈合反应和包膜挛缩的命运。
