Department of Microbiology and Molecular Genetics, IMRIC, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.
Trends Genet. 2017 Sep;33(9):594-603. doi: 10.1016/j.tig.2017.06.006. Epub 2017 Jul 8.
Recent studies show that nuclear RNase P is linked to chromatin structure and function. Thus, variants of this ribonucleoprotein (RNP) complex bind to chromatin of small noncoding RNA genes; integrate into initiation complexes of RNA polymerase (Pol) III; repress histone H3.3 nucleosome deposition; control tRNA and PIWI-interacting RNA (piRNA) gene clusters for genome defense; and respond to Werner syndrome helicase (WRN)-related replication stress and DNA double-strand breaks (DSBs). Likewise, the related RNase MRP and RMRP-TERT (telomerase reverse transcriptase) are implicated in RNA-dependent RNA polymerization for chromatin silencing, whereas the telomerase carries out RNA-dependent DNA polymerization for telomere lengthening. Remarkably, the four RNPs share several protein subunits, including two Alba-like chromatin proteins that possess DEAD-like and ATPase motifs found in chromatin modifiers and remodelers. Based on available data, RNase P and related RNPs act in transition processes of DNA to RNA and vice versa and connect these processes to genome preservation, including replication, DNA repair, and chromatin remodeling.
最近的研究表明，核核糖核酸酶 P 与染色质结构和功能有关。因此，这种核糖核蛋白（RNP）复合物的变体与小非编码 RNA 基因的染色质结合；整合到 RNA 聚合酶（Pol）III 的起始复合物中；抑制组蛋白 H3.3 核小体沉积；控制 tRNA 和 PIWI 相互作用 RNA（piRNA）基因簇以进行基因组防御；并响应 Werner 综合征解旋酶（WRN）相关的复制应激和 DNA 双链断裂（DSB）。同样，相关的核糖核酸酶 MRP 和 RMRP-TERT（端粒酶逆转录酶）参与 RNA 依赖性 RNA 聚合以实现染色质沉默，而端粒酶则进行 RNA 依赖性 DNA 聚合以延长端粒。值得注意的是，这四种 RNP 共享几个蛋白亚基，包括两种具有 DEAD 样和 ATPase 基序的 Alba 样染色质蛋白，这些基序存在于染色质修饰因子和重塑因子中。基于现有数据，核糖核酸酶 P 和相关的 RNP 在 DNA 到 RNA 和反之亦然的转化过程中发挥作用，并将这些过程与基因组保存联系起来，包括复制、DNA 修复和染色质重塑。
