Ministry of Education Key Laboratory for Avian Preventive Medicine, Yangzhou University, Yangzhou, Jiangsu Province, China; Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu Province, China; Department of Microbiology, Faculty of Veterinary Medicine, University of Khartoum, Khartoum North 13314, Sudan.
Ministry of Education Key Laboratory for Avian Preventive Medicine, Yangzhou University, Yangzhou, Jiangsu Province, China; Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu Province, China.
Virus Res. 2017 Jun 15;238:253-257. doi: 10.1016/j.virusres.2017.07.007. Epub 2017 Jul 8.
Avian leukosis virus (ALV) is an avian oncogenic retrovirus that can induce virus-associated neoplasia and causes great economic loss in poultry industry. It is known that the capsid antigen p27 is the group-specific antigen that is highly conserved among all ALV subgroups, and is the most abundant immunogenic viral protein. In the present study, five overlapping fragments (GST- p27-F1/2, GST- p27-F2-1/2/3) of ALV-p27 were subjected to Western blotting analysis using a monoclonal antibody (5D3) against ALV-p27 to identify the epitope. The result showed that the epitope recognized by 5D3 is located within 173-240 amino acid of the ALV-p27 protein. For precise mapping of this epitope, a set of overlapping peptides were synthesized. Indirect enzyme linked immunosorbent assay (ELISA) revealed that CFRQKSQPDI motif was the minimal fragment recognized by 5D3, so this motif represented a linear B-cell epitope of ALV-p27. Homology analysis indicated that 5D3 defined epitope is highly conserved among ALV strains. The identified epitope might be useful in clinical applications and as a tool for further study of the structure and function of ALV-p27.
禽白血病病毒（ALV）是一种禽类致癌逆转录病毒，可诱导病毒相关性肿瘤，并给家禽养殖业造成巨大的经济损失。众所周知，囊膜蛋白 p27 是 ALV 各亚群中具有高度保守性的群特异性抗原，也是最丰富的免疫原性病毒蛋白。在本研究中，使用针对 ALV-p27 的单克隆抗体（5D3）进行 Western blot 分析，鉴定了 ALV-p27 的五个重叠片段（GST- p27-F1/2、GST- p27-F2-1/2/3）的表位。结果表明，5D3 识别的表位位于 ALV-p27 蛋白的 173-240 个氨基酸内。为了精确绘制这个表位，我们合成了一组重叠肽。间接酶联免疫吸附试验（ELISA）表明，CFRQKSQPDI 基序是 5D3 识别的最小片段，因此这个基序代表了 ALV-p27 的线性 B 细胞表位。同源性分析表明，5D3 定义的表位在 ALV 株中高度保守。所鉴定的表位可能在临床应用中有用，并可作为进一步研究 ALV-p27 结构和功能的工具。
