J亚群禽白血病病毒gp85蛋白新B细胞表位的鉴定及其在疾病诊断中的应用
Identification of novel B-cell epitope in gp85 of subgroup J avian leukosis virus and its application in diagnosis of disease.
作者信息
Qian Kun, Tian Xue, Shao Hongxia, Ye Jianqiang, Yao Yongxiu, Nair Venugopal, Qin Aijian
机构信息
Ministry of Education Key Lab for Avian Preventive Medicine, College of Veterinary Medicine, Yangzhou University, No.12 East Wenhui Road, Yangzhou, Jiangsu, 225009, People's Republic of China.
The International Joint Laboratory for Cooperation in Agriculture and Agricultural Product Safety, Ministry of Education, Yangzhou University, 225009, Yangzhou, People's Republic of China.
出版信息
BMC Vet Res. 2018 Sep 26;14(1):295. doi: 10.1186/s12917-018-1622-x.
BACKGROUND
The gp85 is the main envelope protein of avian leukosis subgroup J (ALV-J) involved in virus neutralization. Here, we mapped the epitope in ALV-J gp85 by ELISA using synthetic peptides and developed epitope based diagnostic methods for ALV-J infection.
RESULTS
The results revealed that monoclonal antibody (mAb) JE9 recognized WDPQEL motif, which was highly conserved in gp85 among different ALV-J strains by homology analysis. Moreover, after evaluation with two hundred and forty sera samples obtained from different chicken farms, the epitope-based peptide ELISA had much higher sensitivity than commercial ELISA kit for antibody detection of ALV-J.
CONCLUSIONS
A novel B-cell epitope recognized by the mAb JE9 was identified. The developed peptide-ELISA based on this novel B-cell epitope could be useful in laboratory viral diagnosis, routine surveillance in chicken farms, and also in understanding the pathogenesis of ALV-J.
背景
gp85是禽白血病J亚群（ALV-J）的主要囊膜蛋白，参与病毒中和反应。在此，我们利用合成肽通过酶联免疫吸附测定（ELISA）绘制了ALV-J gp85中的表位，并开发了基于表位的ALV-J感染诊断方法。
结果
结果显示，单克隆抗体（mAb）JE9识别WDPQEL基序，通过同源性分析发现该基序在不同ALV-J毒株的gp85中高度保守。此外，在用从不同养鸡场获得的240份血清样本进行评估后，基于表位的肽ELISA在检测ALV-J抗体方面比商业ELISA试剂盒具有更高的灵敏度。
结论
鉴定出一种可被单克隆抗体JE9识别的新型B细胞表位。基于这种新型B细胞表位开发的肽ELISA可用于实验室病毒诊断、养鸡场的常规监测，也有助于理解ALV-J的发病机制。
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