Li Xiaofei, Qin Liting, Zhu Haibo, Sun Yingjun, Cui Xuezhi, Gao Yadong, Qi Xiaole, Wang Yongqiang, Gao Honglei, Gao Yulong, Wang Xiaomei
Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Nan Gang District, Harbin, Heilongjiang Province, People's Republic of China.
New Hope Liuhe Co., Ltd., Qingdao, China.
Arch Virol. 2016 Oct;161(10):2871-7. doi: 10.1007/s00705-016-2971-z. Epub 2016 Jul 20.
Avian leukosis virus (ALV) is an avian oncogenic retrovirus that can induce various clinical tumors. The capsid protein P27 is the group-specific antigen of ALV and has many viral antigen sites that are easy to detect. In this study, we produced a monoclonal antibody (mAb), 3A9, that is specific for the P27 protein. A series of partially overlapping peptides were screened to define (181)PPSAR(185) as the minimal linear epitope recognized by mAb 3A9. The identified epitope could be recognized by chicken anti-ALV and mouse anti-ALV P27 sera. The epitope was highly conserved among a number of ALV-A, ALV-B and ALV-J strains. MAb 3A9 might be a valuable tool for the development of new immunodiagnostic approaches for ALV, and the defined linear epitope might help further our understanding of the antigenic structure of the P27 protein.
禽白血病病毒（ALV）是一种可诱发多种临床肿瘤的禽类致癌逆转录病毒。衣壳蛋白P27是ALV的群特异性抗原，具有许多易于检测的病毒抗原位点。在本研究中，我们制备了一种针对P27蛋白的单克隆抗体（mAb）3A9。筛选了一系列部分重叠的肽段，以确定（181）PPSAR（185）为mAb 3A9识别的最小线性表位。所鉴定的表位可被鸡抗ALV和小鼠抗ALV P27血清识别。该表位在许多ALV-A、ALV-B和ALV-J毒株中高度保守。mAb 3A9可能是开发ALV新免疫诊断方法的有价值工具，所确定的线性表位可能有助于进一步了解P27蛋白的抗原结构。
