Sun Xiaolin, Wei Haiying, Young Dominique E, Bein Keith J, Smiley-Jewell Suzette M, Zhang Qi, Fulgar Ciara Catherine B, Castañeda Alejandro R, Pham Alexa K, Li Wei, Pinkerton Kent E
Shandong University, Biomedical Engineering Institute, School of Control Science and Engineering, Jinan, China; University of California, Davis, Center for Health and the Environment, USA.
Shanxi University, College of Environmental and Resource Sciences, Taiyuan, China; University of California, Davis, Center for Health and the Environment, USA.
Toxicol Lett. 2017 Aug 15;278:1-8. doi: 10.1016/j.toxlet.2017.07.853. Epub 2017 Jul 8.
Airborne particulate matter (PM) is associated with adverse cardiorespiratory effects. To better understand source-orientated PM toxicity, a comparative study of the biological effects of fine PM (diameter≤2.5μm, PM) collected during the winter season from Shanxi Province, China, and the Central Valley, California, United States, was conducted. The overarching hypothesis for this study was to test whether the chemical composition of PM on an equal mass basis from two urban areas, one in China and one in California, can lead to significantly different effects of acute toxicity and inflammation in the lungs of healthy young mice. Male, 8-week old BALB/C mice received a single 50μg dose of vehicle, Taiyuan PM or Sacramento PM by oropharyngeal aspiration and were sacrificed 24h later. Bronchoalveolar lavage, ELISA and histopathology were performed along with chemical analysis of PM composition. Sacramento PM had a greater proportion of oxidized organic material, significantly increased neutrophil numbers and elevated CXCL-1 and TNF-α protein levels compared to the Taiyuan PM. The findings suggest that Sacramento PM was associated with a greater inflammatory response compared to that of Taiyuan PM that may be due to a higher oxidice. Male, 8-week old BALB/C mice received a single 50μg dose of vehicle, Taiyuan PM or Sacramento PM by oropharyngeal aspiration and were sacrificed 24h later. Bronchoalveolar lavage, ELISA and histopathology were performed along with chemical analysis of PM composition. Sacramento PM had a greater proportion of oxidized organic material, significantly increased neutrophil numbers and elevated CXCL-1 and TNF-α protein levels compared to the Taiyuan PM. The findings suggest that Sacramento PM was associated with a greater inflammatory response compared to that of Taiyuan PM that may be due to a higher oxidized state of organic carbon and copper content.
空气中的颗粒物（PM）与不良的心肺效应相关。为了更好地理解源导向的PM毒性，对冬季在中国山西省和美国加利福尼亚州中央谷地采集的细颗粒物（直径≤2.5μm，PM）的生物学效应进行了一项比较研究。本研究的总体假设是测试来自中国一个城市地区和加利福尼亚州一个城市地区的等质量基础上的PM化学成分是否会导致健康年轻小鼠肺部急性毒性和炎症的显著不同影响。8周龄雄性BALB/C小鼠通过口咽吸入接受单次50μg剂量的赋形剂、太原PM或萨克拉门托PM，并在24小时后处死。进行支气管肺泡灌洗、酶联免疫吸附测定（ELISA）和组织病理学检查以及PM成分的化学分析。与太原PM相比，萨克拉门托PM中氧化有机物质的比例更高，中性粒细胞数量显著增加，CXCL-1和肿瘤坏死因子-α（TNF-α）蛋白水平升高。研究结果表明，与太原PM相比，萨克拉门托PM与更大的炎症反应相关，这可能是由于有机碳的氧化态和铜含量较高所致。8周龄雄性BALB/C小鼠通过口咽吸入接受单次50μg剂量的赋形剂、太原PM或萨克拉门托PM，并在24小时后处死。进行支气管肺泡灌洗、酶联免疫吸附测定（ELISA）和组织病理学检查以及PM成分的化学分析。与太原PM相比，萨克拉门托PM中氧化有机物质的比例更高，中性粒细胞数量显著增加，CXCL-1和肿瘤坏死因子-α（TNF-α）蛋白水平升高。研究结果表明，与太原PM相比，萨克拉门托PM与更大的炎症反应相关，这可能是由于有机碳的氧化态和铜含量较高所致。
