El Khoury Rachelle, Atoui Ali, Mathieu Florence, Kawtharani Hiba, El Khoury Anthony, Maroun Richard G, El Khoury Andre
Laboratoire de Mycologie et Sécurité Alimentaire (LMSA), Centre d'analyse et de Recherche (CAR), Campus des Sciences et Technologie, Université Saint-Joseph, Dekwaneh-Beyrouth 1104-2020, Lebanon.
Laboratoire de Génie Chimique, Université de Toulouse, Centre National de Recherche Scientifique (CNRS), Institut National Polytechnique de Toulouse (INPT), Université Paul Sabatier (UPS), Toulouse 31326, France.
Antioxidants (Basel). 2017 Jul 9;6(3):44. doi: 10.3390/antiox6030044.
This study is intended to prevent ochratoxin A (OTA) production by S402 using essential oils (EOs) and total phenolic compounds extracted from plants and herbs. The EOs used in this study are the following: bay leaves, cumin, fenugreek, melissa, mint, and sage. As for the phenolic compounds, they were extracted from bay leaves, cumin, fenugreek, melissa, mint, sage, anise, chamomile, fennel, rosemary, and thyme. The experiments were conducted on Synthetic Grape Medium (SGM) medium at 28 °C for 4 days. OTA was extracted from the medium with methanol and quantified using HPLC (High Performance Liquid Chromatography). Results showed that EOs had a greater impact than the total phenolic extracts on the OTA production. Reduction levels ranged between 25% (sage) and 80% (melissa) for the EOs at 5 µL mL, and 13% (thyme) and 69% (mint) for the phenolic extracts. Although they did not affect the growth of , total phenolic extracts and EOs were capable of partially reducing OTA production. Reduction levels depended on the nature of the plants and the concentration of the EOs. Reducing OTA with natural extracts could be a solution to prevent OTA production without altering the fungal growth, thus preserving the natural microbial balance.
本研究旨在利用从植物和草药中提取的精油（EOs）和总酚类化合物来防止S402产生赭曲霉毒素A（OTA）。本研究中使用的精油如下：月桂叶、小茴香、胡芦巴、蜜蜂花、薄荷和鼠尾草。至于酚类化合物，它们是从月桂叶、小茴香、胡芦巴、蜜蜂花、薄荷、鼠尾草、茴芹、洋甘菊、茴香、迷迭香和百里香中提取的。实验在合成葡萄培养基（SGM）上于28℃进行4天。用甲醇从培养基中提取OTA，并使用高效液相色谱法（HPLC）进行定量。结果表明，精油对OTA产生的影响比总酚提取物更大。在5μL/mL时，精油的减少水平在25%（鼠尾草）至80%（蜜蜂花）之间，酚类提取物的减少水平在13%（百里香）至69%（薄荷）之间。尽管它们不影响S402的生长，但总酚提取物和精油能够部分降低OTA的产生。减少水平取决于植物的性质和精油的浓度。用天然提取物减少OTA可能是一种在不改变真菌生长的情况下防止OTA产生的解决方案，从而保持自然微生物平衡。
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