Lee Jihwan, Cho Hyunah, Song Dongcheol, Chang Seyeon, An Jaewoo, Nam Jeonghun, Lee Byoungkon, Kim Sowoong, Kim Woo Kyun, Cho Jinho
Department of Poultry Science, University of Georgia, Athens, GA 30602, USA.
Department of Animal Science, Chungbuk National University, Cheongju 28644, Republic of Korea.
Animals (Basel). 2023 Jul 11;13(14):2266. doi: 10.3390/ani13142266.
The objective of this study was to investigate the effects of toxin binders on broiler breeders fed an ochratoxin A (OTA)-contaminated diet. A total of 60 45-week-old female Arbor Acres broiler breeder birds with an initial body weight of 3.65 ± 0.35 kg were randomly divided into 6 treatment groups, with 10 replicates per group and 1 bird per replicate. The trial was conducted for 9 weeks (including 1 week of adaptation). Feed additive 1 (FA1) was composed of clay minerals (85% bentonite and 12% clinoptilolite) with 3% charcoal. FA2 was composed of clay minerals (66.1% aluminosilicates) with natural components (0.8% artichoke and rosemary plant extracts), 7% yeast extract, 0.5% beta-glucans, and 25.6% carriers. The dietary treatment groups were as follows: (1) birds fed an OTA-free basal diet (Negative Control; NC); (2) lipopolysaccharide (LPS)-challenged birds fed a diet including OTA (4 mg/kg) (Positive Control, PC); (3) the PC with 0.05% FA1 (Treatment 1, T1); (4) the PC with 0.10% FA1 (Treatment 2, T2); (5) the PC with 0.10% FA2 (Treatment 3, T3); and (6) the PC with 0.20% FA2 (Treatment 4, T4). The LPS challenge (an intramuscular injection of 1 mg O55:B5 LPS per kg of body weight) was performed on the first day of the experiment. The results of this experiment show that the PC treatment negatively affected ( < 0.05) egg production, hatchability, Haugh unit, bone mineralization, relative organ weight (abdominal fat, liver), the levels of glutamic oxaloacetic transaminase (GOT), high-density lipoprotein (HDL), and total cholesterol in the blood, and OTA accumulation in the liver compared with the NC. However, supplementation with toxin binders mitigated ( < 0.05) the negative effects of the OTA. Specifically, supplementation with 0.10% FA1 and 0.10% FA2 increased ( < 0.05) eggshell strength by week 4, and the Haugh unit and bone mineralization (phosphorous) by week 8, while decreasing ( < 0.05) the relative weight of the liver and the levels of GOT and HDL in the blood. Supplementation with 0.10% FA2 led to greater improvements in various parameters, including laying performance and bone mineralization, than the other treatments. In conclusion, toxin binders with or without natural components can be effective tools in the mitigation of OTA-induced problems due to their synergistic effects.
本研究的目的是调查毒素吸附剂对采食受赭曲霉毒素A（OTA）污染日粮的肉种鸡的影响。总共60只45周龄的雌性爱拔益加肉种鸡，初始体重为3.65±0.35千克，被随机分为6个处理组，每组10个重复，每个重复1只鸡。试验进行9周（包括1周适应期）。饲料添加剂1（FA1）由粘土矿物（85%膨润土和12%斜发沸石）与3%木炭组成。FA2由粘土矿物（66.1%硅铝酸盐）与天然成分（0.8%洋蓟和迷迭香叶提取物）、7%酵母提取物、0.5%β-葡聚糖和25.6%载体组成。日粮处理组如下：（1）采食不含OTA的基础日粮的鸡（阴性对照；NC）；（2）经脂多糖（LPS）攻毒且采食含OTA（4毫克/千克）日粮的鸡（阳性对照，PC）；（3）添加0.05%FA1的PC组（处理1，T1）；（4）添加0.10%FA1的PC组（处理2，T2）；（5）添加0.10%FA2的PC组（处理3，T3）；（6）添加0.20%FA2的PC组（处理4，T4）。在实验第一天进行LPS攻毒（每千克体重肌肉注射1毫克O55:B5 LPS）。本实验结果表明，与NC组相比，PC处理对产蛋性能、孵化率、哈氏单位、骨矿化、相对器官重量（腹脂、肝脏）、血液中谷氨酸草酰乙酸转氨酶（GOT）、高密度脂蛋白（HDL）和总胆固醇水平以及肝脏中OTA蓄积产生负面影响（P<0.05）。然而，添加毒素吸附剂减轻了（P<0.05）OTA的负面影响。具体而言，添加0.10%FA1和0.10%FA2在第4周时提高了（P<0.05）蛋壳强度，在第8周时提高了哈氏单位和骨矿化（磷），同时降低了（P<0.05）肝脏相对重量以及血液中GOT和HDL水平。与其他处理相比，添加0.10%FA2在包括产蛋性能和骨矿化等各项参数上带来了更大改善。总之，含或不含天然成分的毒素吸附剂因其协同效应可成为减轻OTA诱导问题的有效工具。
