Slade William O, Ray W Keith, Hildreth Sherry B, Winkel Brenda S J, Helm Richard F
Department of Biological Sciences, Virginia Tech, Blacksburg, VA 24061, USA.
Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061, USA.
Proteomes. 2017 Jul 10;5(3):16. doi: 10.3390/proteomes5030016.
Auxin is involved in many aspects of root development and physiology, including the formation of lateral roots. Improving our understanding of how the auxin response is mediated at the protein level over time can aid in developing a more complete molecular framework of the process. This study evaluates the effects of exogenous auxin treatment on the root proteome after exposure of young seedlings to auxin for 8, 12, and 24 h, a timeframe permitting the initiation and full maturation of individual lateral roots. Root protein extracts were processed to peptides, fractionated using off-line strong-cation exchange, and analyzed using ultra-performance liquid chromatography and data independent acquisition-based mass spectrometry. Protein abundances were then tabulated using label-free techniques and evaluated for significant changes. Approximately 2000 proteins were identified during the time course experiment, with the number of differences between the treated and control roots increasing over the 24 h time period, with more proteins found at higher abundance with exposure to auxin than at reduced abundance. Although the proteins identified and changing in levels at each time point represented similar biological processes, each time point represented a distinct snapshot of the response. Auxin coordinately regulates many physiological events in roots and does so by influencing the accumulation and loss of distinct proteins in a time-dependent manner. Data are available via ProteomeXchange with the identifier PXD001400.
生长素参与根发育和生理的许多方面，包括侧根的形成。随着时间的推移，提高我们对生长素反应在蛋白质水平上如何介导的理解，有助于建立一个更完整的该过程分子框架。本研究评估了将幼苗暴露于生长素8小时、12小时和24小时后，外源生长素处理对根蛋白质组的影响，该时间范围允许单个侧根的起始和完全成熟。将根蛋白提取物处理成肽段，通过离线强阳离子交换进行分离，并用超高效液相色谱和基于数据非依赖采集的质谱进行分析。然后使用无标记技术将蛋白质丰度制成表格，并评估其显著变化。在时间进程实验中鉴定出约2000种蛋白质，处理根和对照根之间的差异数量在24小时内增加，与生长素处理后丰度降低相比，发现更多蛋白质在生长素处理后丰度增加。尽管在每个时间点鉴定出的蛋白质及其水平变化代表相似的生物学过程，但每个时间点代表了反应的一个独特快照。生长素以时间依赖性方式协调调节根中的许多生理事件，通过影响不同蛋白质的积累和损失来实现。数据可通过ProteomeXchange获得，标识符为PXD001400。
