Torsello Monica, Animini Margherita, Gualandi Chiara, Perut Francesca, Pollicino Antonino, Boi Cristiana, Focarete Maria Letizia
Department of Chemistry "G. Ciamician" and INSTM (National Interuniversity Consortium of Materials Science and Technology) UdR of Bologna, University of Bologna, Via Selmi 2, 40126 Bologna, Italy.
Interdepartmental Center for Industrial Research on Advanced Applications in Mechanical Engineering and Materials Technology (CIRI-MAM), University of Bologna, Viale Risorgimento 2, 40136 Bologna, Italy.
Membranes (Basel). 2024 Sep 26;14(10):206. doi: 10.3390/membranes14100206.
Electrospun regenerated cellulose (RC) nanofiber membranes were prepared starting from cellulose acetate (CA) with different degrees of substitution. The process was optimized to obtain continuous and uniformly sized CA fibers. After electrospinning, the CA membranes were heat-treated to increase their tensile strength before deacetylation to obtain regenerated cellulose (RC). Affinity membranes were obtained by functionalization, exploiting the hydroxyl groups on the cellulose backbone. 1,4-Butanediol-diglycidyl ether was used to introduce epoxy groups onto the membrane, which was further bioconjugated with the anti-CD63 antibody targeting the tetraspanin CD63 on the extracellular vesicle membrane surface. The highest ligand density was obtained with an anti-CD63 antibody concentration of 6.4 µg/mL when bioconjugation was performed in carbonate buffer. The resulting affinity membrane was tested for the adsorption of extracellular vesicles (EVs) from human platelet lysate, yielding a very promising binding capacity above 10 mg/mL and demonstrating the suitability of this approach.
从具有不同取代度的醋酸纤维素（CA）出发制备了静电纺丝再生纤维素（RC）纳米纤维膜。对该工艺进行了优化，以获得连续且尺寸均匀的CA纤维。静电纺丝后，在脱乙酰化之前对CA膜进行热处理以提高其拉伸强度，从而获得再生纤维素（RC）。通过功能化利用纤维素主链上的羟基获得亲和膜。使用1,4 - 丁二醇二缩水甘油醚将环氧基团引入膜中，该膜进一步与靶向细胞外囊泡膜表面四跨膜蛋白CD63的抗CD63抗体进行生物共轭。当在碳酸盐缓冲液中进行生物共轭时，抗CD63抗体浓度为6.4 µg/mL时获得了最高的配体密度。对所得的亲和膜进行了从人血小板裂解物中吸附细胞外囊泡（EVs）的测试，其结合能力超过10 mg/mL，前景十分可观，证明了该方法的适用性。
