Forestry College, Fujian Agriculture and Forestry University, Fuzhou, 350002, P. R. China.
Chinese Fir Engineering Technology Research Center of the State Forestry Administration, Fuzhou, 350002, P. R. China.
Sci Rep. 2017 Jul 11;7(1):5046. doi: 10.1038/s41598-017-05295-z.
In order to ascertain the optimal hormone combination for Chinese fir (Cunninghamia lanceolata) leaf cuttings, an L(4) orthogonal test of 6-benzylaminopurine (6-BA), 1-naphthaleneacetic acid (NAA), and indole-3-butyric acid (IBA) (0, 10, 30, or 50 mg · L of each exogenous hormone) immersion for 5, 10, 15, or 20 min was conducted. Callus initiation rate and rooting promotion rate were mainly affected by treatment time, root length increase by 6-BA concentration, and bud germination rate and plantlet formation rate by NAA concentration. The expected optimal combination for callus initiation rate was 50 mg · L 6-BA + 0 mg · L NAA + 30 mg · L IBA + 10 min; for rooting promotion rate, it was 0-10 mg · L 6-BA + 10 mg · L NAA + 30 mg · L IBA + 20 min; for bud germination rate, it was 50 mg · L 6-BA + 0-10 mg · L NAA + 0-30 mg · L IBA + 20 min; and for seedling formation rate for No. 4, it was 10 mg · L 6-BA + 10 mg · L NAA + 0 mg · L IBA + 20 min. Light microscopy image analysis revealed that a cluster of primordial cells was produced 40 days after cutting, and mastoid cells developed into peninsula cells in calli that were cultured for 50 days.
为了确定杉木叶片扦插的最佳激素组合，采用 6-苄基氨基嘌呤（6-BA）、1-萘乙酸（NAA）和吲哚丁酸（IBA）的 L(4)正交试验（每种外源激素浓度为 0、10、30 或 50mg·L），浸泡 5、10、15 或 20min。愈伤组织诱导率和生根促进率主要受处理时间的影响，根长增加受 6-BA 浓度的影响，芽萌发率和植株形成率受 NAA 浓度的影响。愈伤组织诱导率的预期最佳组合为 50mg·L 6-BA+0mg·L NAA+30mg·L IBA+10min；生根促进率为 0-10mg·L 6-BA+10mg·L NAA+30mg·L IBA+20min；芽萌发率为 50mg·L 6-BA+0-10mg·L NAA+0-30mg·L IBA+20min；第 4 号苗的成苗率为 10mg·L 6-BA+10mg·L NAA+0mg·L IBA+20min。光镜图像分析表明，在切割后 40 天产生了一团原始细胞，在培养 50 天的愈伤组织中，多角体细胞发育成半岛细胞。
