Ji Yuan, Zhu Liming, Hao Zhaodong, Su Shunde, Zheng Xueyan, Shi Jisen, Zheng Renhua, Chen Jinhui
Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.
School of Life Science, Huaiyin Normal University, Jiangsu, China.
Front Bioeng Biotechnol. 2022 Mar 7;10:854130. doi: 10.3389/fbioe.2022.854130. eCollection 2022.
(Lamb.)Hook is an important economic timber tree in China. However, its genome characteristics have not been extensively assessed. To better understand its genome information, the bacterial artificial chromosome (BAC) library of chinese fir was constructed. A total of 422 BAC clones were selected and divided into 10 pools and sequenced, and with an average insert size of 121 kb, ranging from 97 to 145 kb. A total of 61,902,523 bp of reference sequences were sequenced and assembled, and based on an estimated genome size of 11.6 Gb for Chinese fir, the BAC library was estimated to have a total coverage of 0.53% genome equivalents. Bioinformatics analyses were also performed for repeated sequences, tRNAs, coding gene prediction, and functional annotation. The results of this study provide insights into the brief structure of the Chinese fir genome and has generated gene data that will facilitate molecular investigations on the mechanisms underlying tree growth.
（兰姆.）胡克是中国一种重要的经济用材树种。然而，其基因组特征尚未得到广泛评估。为了更好地了解其基因组信息，构建了杉木细菌人工染色体（BAC）文库。共选择了422个BAC克隆，分为10个池并进行测序，平均插入片段大小为121 kb，范围为97至145 kb。共测序和组装了61,902,523 bp的参考序列，基于杉木估计基因组大小为11.6 Gb，估计该BAC文库的总覆盖率为0.53%基因组当量。还对重复序列、tRNA、编码基因预测和功能注释进行了生物信息学分析。本研究结果为杉木基因组的简要结构提供了见解，并产生了基因数据，这将有助于对树木生长潜在机制进行分子研究。
