Popoca-Ursino Elida C, Martínez-Ocampo Fernando, Dantán-González Edgar, Sánchez-Salinas Enrique, Ortiz-Hernández Ma Laura
Laboratorio de Investigaciones Ambientales, Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, C.P. 62209, Cuernavaca, Morelos, Mexico.
Biodegradation. 2017 Dec;28(5-6):351-367. doi: 10.1007/s10532-017-9801-1. Epub 2017 Jul 11.
Through the use of an enrichment technique, we isolated from the agricultural soils of Morelos in central México a strain of Burkholderia zhejiangensis identified as CEIB S4-3, it's could use the pesticide methyl parathion (MP) as the only source of carbon and degrade completely p-nitrophenol (PNP). For more efficient MP and PNP degradation by the CEIB S4-3 strain, the absence of an extra carbon source, a large inoculum and an MP concentration up to 50 mg/l are required. Sequence and annotation analysis of the draft genome, showed presence of mpd functional gene, which was expressed and its activity on the MP was confirmed. Additionally, the genes coding for enzymes in the benzoquinone pathway (conducted by Gram-negative bacteria) and the benzenotriol pathway (conducted by Gram-positive bacteria) were found, which was corroborated by identification of intermediary metabolites by HPLC. Thus, we propose that B. zhejiangensis CEIB S4-3 uses both degradation pathways.
通过一种富集技术，我们从墨西哥中部莫雷洛斯州的农业土壤中分离出一株被鉴定为浙江伯克霍尔德氏菌CEIB S4-3的菌株，它能够利用农药甲基对硫磷（MP）作为唯一碳源，并能完全降解对硝基苯酚（PNP）。为了使CEIB S4-3菌株更高效地降解MP和PNP，需要不存在额外碳源、使用大量接种物且MP浓度高达50 mg/l。对基因组草图的序列和注释分析表明存在mpd功能基因，该基因得到表达并证实了其对MP的活性。此外，还发现了编码苯醌途径（由革兰氏阴性菌进行）和苯三酚途径（由革兰氏阳性菌进行）中酶的基因，通过高效液相色谱法鉴定中间代谢产物对此进行了证实。因此，我们提出浙江伯克霍尔德氏菌CEIB S4-3利用这两种降解途径。
