Baazm Maryam, Mashayekhi Farideh Jalali, Babaie Saeid, Bayat Parvindokht, Beyer Cordian, Zendedel Adib
Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
Department of Biochemistry and Genetics, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
In Vitro Cell Dev Biol Anim. 2017 Sep;53(8):752-758. doi: 10.1007/s11626-017-0172-z. Epub 2017 Jul 11.
Spermatongonial stem cells (SSCs) are unique testis cells that are able to proliferate, differentiate, and transmit genetic information to the next generation. However, the effect of different Sertoli cell types on the expression of specific SSC genes is not yet well understood. In this study, we compare the in vitro effect of adult Sertoli cells, embryonic Sertoli cells, and TM4 (a Sertoli cell line) as feeder layers on the expression of SSC genes. SSCs were isolated from the testis of adult male mice and purified by differential plating. Following enrichment, SSCs were cultivated for 1 and 2 wk in the presence of various feeders. The expression of SSC-specific genes (Mvh, ZBTB, and c-kit) was evaluated by real-time polymerase chain reaction. Our results revealed that expression of the specific SSC genes was significantly higher in the embryonic Sertoli cells after 1 and 2 wk compared to the adult Sertoli cells and the TM4 group. Our finding suggest that co-culturing of SSCs with embryonic Sertoli cells is helpful for in vitro cultivation of SSCs and might improve the self-renewal of these stem cells.
精原干细胞（SSCs）是睾丸中独特的细胞，能够增殖、分化并将遗传信息传递给下一代。然而，不同类型的支持细胞对特定SSC基因表达的影响尚未完全明确。在本研究中，我们比较了成年支持细胞、胚胎支持细胞和TM4（一种支持细胞系）作为饲养层对SSC基因表达的体外影响。从成年雄性小鼠睾丸中分离出SSCs，并通过差异铺板进行纯化。富集后，将SSCs在各种饲养层存在的情况下培养1周和2周。通过实时聚合酶链反应评估SSC特异性基因（Mvh、ZBTB和c-kit）的表达。我们的结果显示，与成年支持细胞和TM4组相比，胚胎支持细胞在培养1周和2周后，特定SSC基因的表达显著更高。我们的发现表明，SSCs与胚胎支持细胞共培养有助于SSCs的体外培养，并可能改善这些干细胞的自我更新能力。
